Inferring spatial organization of bonds within adhesion clusters by exploiting fluctuations of soft interfaces

Smith AS, Fenz SF, Sengupta K (2010)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2010

Journal

EPL - Europhysics Letters Institute of Physics: EPL

Publisher: EPL ASSOCIATION, EUROPEAN PHYSICAL SOCIETY

Book Volume: 89

Article Number: 28003

Journal Issue: 2

URI: http://iopscience.iop.org/article/10.1209/0295-5075/89/28003/meta

DOI: 10.1209/0295-5075/89/28003

Abstract

Detecting the organization of bonds within adhesion domains connecting two interacting membranes is, at present, extremely challenging. Herein we present a technique, based on Reflection Interference Contrast Microscopy, which uses spontaneous thermal fluctuations of a soft interface as a tool to identify the organization of specific ligand-receptor bonds. The key is a time-resolved analysis of micro-interferometric data that systematically quantifies fluctuations and enables the detection of their suppression due to the formation of bonds, which, in turn, allows the identification of the bond organization without the use of fluorescent labelling. The identification of a new type of bond organization characterized by sparsely distributed bonds, as well as detection of pinning centres of nanometric size is presented. Copyright (C) EPLA, 2010

Authors with CRIS profile

Ana-Suncana Smith Physics Underlying Life Science (PULS)

Additional Organisation(s)

Exzellenz-Cluster Engineering of Advanced Materials (EAM)

Related research project(s)

None - None

Involved external institutions

Forschungszentrum Jülich / Research Centre Jülich (FZJ) DE Germany (DE) Aix-Marseille University / Aix-Marseille Université FR France (FR)

How to cite

APA:

Smith, A.-S., Fenz, S.F., & Sengupta, K. (2010). Inferring spatial organization of bonds within adhesion clusters by exploiting fluctuations of soft interfaces. EPL - Europhysics Letters, 89(2). https://doi.org/10.1209/0295-5075/89/28003

MLA:

Smith, Ana-Suncana, S. F. Fenz, and Kheya Sengupta. "Inferring spatial organization of bonds within adhesion clusters by exploiting fluctuations of soft interfaces." EPL - Europhysics Letters 89.2 (2010).

BibTeX: Download