Brehmer A, Schrödl F, Neuhuber W (2002)
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2002
Publisher: Wiley-Blackwell
Book Volume: 453
Pages Range: 1-9
Journal Issue: 1
DOI: 10.1002/cne.10358
The study was aimed at the immunohistochemical characterization of myenteric Stach type V neurons of the pig ileum that were not included in the widely used Dogiel classification. So far, this conspicuous population has been defined morphologically on the basis of silver-impregnated specimens only. By using neurofilament immunohistochemistry, type V neurons that occur singly or in aggregates could be identified unequivocally and could be distinguished from other smoothly contoured myenteric neurons, i.e., type II and type IV. Double-labeling immunohistochemistry revealed a number of potentially neuroactive substances or their synthesizing enzymes to be present in type V neurons. Choline acetyltransferase immunoreactivity (-ir) was found in all type V neurons, whereas neuronal nitric oxide synthase was detected in none. Leu-enkephalin-ir was found within 92.3%, somatostatin (SOM)-ir within 91.1%, calcitonin gene-related peptide (CGRP)-ir within 80.6% and metenkephalin-ir within 74.7% of type V neurons. Triple-labeling immunohistochemistry was applied to address the question of a specific chemical coding for myenteric type V neurons. In contrast to other combinations of neuroactive substances/enzymes that were found in both type V and other, nontype V neurons, SOM/CGRP-ir was the only combination observed exclusively within type V neurons. Both substances were colocalized in 79.3% of type V neurons. This colocalization discriminates four-fifths of the type V neurons chemically from both type II neurons (CGRP positive, SOM negative) and type IV neurons (CGRP negative, SOM positive), which both share, at first glance, a similar morphology with type V neurons. These results further support the concept of a close correlation between morphologically defined neuronal type and chemical coding and, it is likely, also function in the enteric nervous system of larger mammals. © 2002 Wiley-Liss, Inc.
APA:
Brehmer, A., Schrödl, F., & Neuhuber, W. (2002). Correlated morphological and chemical phenotyping in myenteric type V neurons of porcine ileum. Journal of Comparative Neurology, 453(1), 1-9. https://doi.org/10.1002/cne.10358
MLA:
Brehmer, Axel, Falk Schrödl, and Winfried Neuhuber. "Correlated morphological and chemical phenotyping in myenteric type V neurons of porcine ileum." Journal of Comparative Neurology 453.1 (2002): 1-9.
BibTeX: Download