Induction of exportin-5 expression during melanoma development supports the cellular behavior of human malignant melanoma cells

Ott C, Linck L, Kremmer E, Meister G, Boßerhoff AK (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Oncotarget Impact Journals

DOI: 10.18632/oncotarget.11410

Abstract

Regulation of gene expression via microRNAs is known to promote the development of many types of cancer. In melanoma, miRNAs are globally up-regulated, and alterations of miRNA-processing enzymes have already been identified. However, mis-regulation of miRNA transport has not been analyzed in melanoma yet. We hypothesized that alterations in miRNA transport disrupt miRNA processing. Therefore, we investigated whether the pre-miRNA transporter Exportin-5 (XPO5) was involved in altered miRNA maturation and functional consequences in melanoma. We found that XPO5 is significantly over-expressed in melanoma compared with melanocytes. We showed enhanced XPO5 mRNA stability in melanoma cell lines which likely contributes to up-regulated XPO5 protein expression. In addition, we identified MEK signaling as a regulator of XPO5 expression in melanoma. Knockdown of XPO5 expression in melanoma cells led to decreased mature miRNA levels and drastic functional changes. Our data revealed that aberrant XPO5 expression is important for the maturation of miRNAs and the malignant behavior of melanoma cells. We suggest that the high abundance of XPO5 in melanoma leads to enhanced survival, proliferation and metastasis and thereby supports the aggressiveness of melanoma.

Authors with CRIS profile

Corinna Ott Lehrstuhl für Biochemie und Molekulare Medizin Lisa Linck-Paulus Lehrstuhl für Biochemie und Molekulare Medizin Anja Katrin Boßerhoff Lehrstuhl für Biochemie und Molekulare Medizin

Additional Organisation(s)

Emil-Fischer-Zentrum (Emil Fischer Center)

Involved external institutions

Universität Regensburg DE Germany (DE) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE)

How to cite

APA:

Ott, C., Linck, L., Kremmer, E., Meister, G., & Boßerhoff, A.K. (2016). Induction of exportin-5 expression during melanoma development supports the cellular behavior of human malignant melanoma cells. Oncotarget. https://doi.org/10.18632/oncotarget.11410

MLA:

Ott, Corinna, et al. "Induction of exportin-5 expression during melanoma development supports the cellular behavior of human malignant melanoma cells." Oncotarget (2016).

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