High-resolution mass spectrometry for thymosins detection and characterization
Cabras T, Iavarone F, Martelli C, Delfino D, Rossetti DV, Inserra I, Manconi B, Desiderio C, Messana I, Hannappel E, Faa G, Castagnola M (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 15 Suppl 1
Pages Range: S191-201
DOI: 10.1517/14712598.2015.1009887
Abstract
The aim of this study was to characterize ? and ? thymosins and their proteoforms in various tissues and bodily fluids by mass spectrometry and to look at their association with a wide variety of pathologies.A top-down proteomic platform based on high-performance liquid chromatography (HPLC) coupled to high-resolution LTQ-Orbitrap mass spectrometry (MS) was applied to the characterization of naturally occurring peptides.In addition to thymosin ?4 (T?4) and ?10 (T?10), several post-translational modifications of both these peptides were identified not only in bodily fluids but also in normal and pathological tissues of different origins. The analysis of tissue specimens allowed the characterization of different C-terminal truncated forms of T?4 and T?10 together with other proteolytic fragments. The sulfoxide derivative of both T?4 and T?10 and the acetylated derivatives at lysine residues of T?4 were also characterized. Different proteoforms of prothymosin ?, parathymosin ?, thymosin ?1 and thymosin ?11 together with diverse proteolytic fragments were identified too.The clinical and prognostic significance and the origin of these proteoforms have to be deeply investigated.
Authors with CRIS profile
Involved external institutions
Università degli Studi di Cagliari
Italy (IT)
Catholic University of the Sacred Heart / Università Cattolica del Sacro Cuore
Italy (IT)
Consiglio Nazionale delle Ricerche (CNR) / National Research Council of Italy
Italy (IT)
Italy (IT)
Catholic University of the Sacred Heart / Università Cattolica del Sacro Cuore
Italy (IT)
Consiglio Nazionale delle Ricerche (CNR) / National Research Council of Italy
Italy (IT)
How to cite
APA:
Cabras, T., Iavarone, F., Martelli, C., Delfino, D., Rossetti, D.V., Inserra, I.,... Castagnola, M. (2015). High-resolution mass spectrometry for thymosins detection and characterization. Expert Opinion on Biological Therapy, 15 Suppl 1, S191-201. https://doi.org/10.1517/14712598.2015.1009887
MLA:
Cabras, Tiziana, et al. "High-resolution mass spectrometry for thymosins detection and characterization." Expert Opinion on Biological Therapy 15 Suppl 1 (2015): S191-201.
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