Callose Biosynthesis Regulates Symplastic Trafficking during Root Development

Vaten A, Dettmer J, Wu S, Stierhof YD, Miyashima S, Yadav SR, Roberts CJ, Campilho A, Bulone V, Lichtenberger R, Lehesranta S, Mahonen AP, Kim JY, Jokitalo E, Sauer N, Scheres B, Nakajima K, Carlsbecker A, Gallagher KL, Helariutta Y (2011)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2011


Publisher: Elsevier (Cell Press)

Book Volume: 21

Pages Range: 1144-1155

Journal Issue: 6

DOI: 10.1016/j.devcel.2011.10.006

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Plant cells are connected through plasmodesmata (PD), membrane-lined channels that allow symplastic movement of molecules between cells. However, little is known about the role of PD-mediated signaling during plant morphogenesis. Here, we describe an Arabidopsis gene, CALS3/GSL12. Gain-of-function mutations in CALS3 result in increased accumulation of callose (beta-1,3-glucan) at the PD, a decrease in PD aperture, defects in root development, and reduced intercellular trafficking. Enhancement of CALS3 expression during phloem development suppressed loss-of-function mutations in the phloem abundant callose synthase, CALS7 indicating that CALS3 is a bona fide callose synthase. CALS3 alleles allowed us to spatially and temporally control the PD aperture between plant tissues. Using this tool, we are able to show that movement of the transcription factor SHORT-ROOT and microRNA1 65 between the stele and the endodermis is PD dependent. Taken together, we conclude that regulated callose biosynthesis at PD is essential for cell signaling.

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Vaten, A., Dettmer, J., Wu, S., Stierhof, Y.-D., Miyashima, S., Yadav, S.R.,... Helariutta, Y. (2011). Callose Biosynthesis Regulates Symplastic Trafficking during Root Development. Developmental Cell, 21(6), 1144-1155.


Vaten, Anne, et al. "Callose Biosynthesis Regulates Symplastic Trafficking during Root Development." Developmental Cell 21.6 (2011): 1144-1155.

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