Vascularization of the Arteriovenous Loop in a Rat Isolation Chamber Model-Quantification of Hypoxia and Evaluation of Its Effects

Yuan Q, Arkudas A, Horch RE, Hammon M, Bleiziffer O, Uder M, Seuß H (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Tissue Engineering: Parts A, B, and C Mary Ann Liebert

DOI: 10.1089/ten.TEA.2017.0262

Abstract

The aim of this study was to analyze the three-dimensional distribution of hypoxia in the arteriovenous (AV) loop model in rats, by examining the distribution of hypoxia-inducible factor-1 alpha (HIF-1?).AV loops were created from the femoral artery and vein of male Lewis rats and an interpositional graft from the contralateral femoral vein. This AV fistula was embedded in a fibrin-filled isolation chamber and subcutaneously implanted into the thigh. The specimens were harvested after 7 days (n = 4), 10 days (n = 5), and 14 days (n = 4). The fibrin clots were stained for lectin, HIF-1?, and ectodysplasin 1 (ED1). The distribution of positive and negative cells was analyzed in three dimensions and at different points in time.The HIF-1?-positive rate increased from the proximity of the central vessel to the distant regions. From day 7 to 10, we noted a decrease in the HIF-1?-positive rate in the proximity of the vessels and an increase in the periphery. A global decrease in positive cells was seen at day 14. HIF-1? and macrophage (ED1) double staining indicated that macrophages accounted for a significant fraction of the cells. Double staining for endothelium (with lectin) demonstrated that no HIF-1? was detectable in well-vascularized areas.In the AV loop model, the HIF-1?-positive cell distribution is highly related to the vascularization process. The onset of rapid vessel outgrowth follows the increase of the HIF-1? rate closely, indicating that HIF-1? may be a driving force for vascularization.

Authors with CRIS profile

Quan Yuan Professur für Plastische Chirurgie und Handchirurgie Andreas Arkudas Medizinische Fakultät Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Matthias Hammon Institute of Radiology Michael Uder Lehrstuhl für Diagnostische Radiologie Hannes Seuß Professur für Plastische Chirurgie und Handchirurgie

How to cite

APA:

Yuan, Q., Arkudas, A., Horch, R.E., Hammon, M., Bleiziffer, O., Uder, M., & Seuß, H. (2017). Vascularization of the Arteriovenous Loop in a Rat Isolation Chamber Model-Quantification of Hypoxia and Evaluation of Its Effects. Tissue Engineering: Parts A, B, and C. https://doi.org/10.1089/ten.TEA.2017.0262

MLA:

Yuan, Quan, et al. "Vascularization of the Arteriovenous Loop in a Rat Isolation Chamber Model-Quantification of Hypoxia and Evaluation of Its Effects." Tissue Engineering: Parts A, B, and C (2017).

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