Bone marrow cells and CD117-positive haematopoietic stem cells promote corneal wound healing
Sel S, Schilling UM, Nass N, Simm A, Garreis F, Knak M, Storsberg J, Kaiser M, Kalinski T, Ehrich D, Bredehorn-Mayr T, Paulsen F (2012)
Publication Type: Journal article
Publication year: 2012
Journal
Book Volume: 90
Pages Range: e367-e373
Journal Issue: 5
DOI: 10.1111/j.1755-3768.2012.02388.x
Abstract
Purpose: The present study was conducted to evaluate the therapeutic effects of topically applied bone marrow (BM) cells and CD117-positive haematopoietic stem (CD117(+)) cells on alkali-induced corneal ulcers. Methods: Bone marrow cells and CD117(+) cells were isolated from syngenic mice and labelled with an intracellular cell tracer. Defined corneal wounds were produced in 89 eyes of syngenic mice and allowed to partially heal in vivo for 6 hr. The alkali-burned eyes were enucleated 6 hr postinjury and randomly divided into three groups. Control group (33 eyes) was incubated with medium only. The treatment groups received either BM cells (30 eyes) or CD117(+) cells (26 eyes) suspended in medium. Re-epithelialization process of corneal defects was qualitatively and quantitatively assessed and statistically analysed. The corneas were examined by histological and immunohistochemical methods. Results: We found that the re-epithelialization of corneal wounds in both treatment groups was significantly accelerated as compared to the control group. During the follow-up period (85 hr), the corneal transparency was comparable in all groups. Morphological investigations of corneas from control and treatment group showed no evident differences in the phenotype of the regenerated epithelium. Additionally, corneas in the treatment groups were devoid of donor-derived BM cells and CD117(+) cells, respectively. Conclusions: This study provides evidence that topical application of BM cells or CD117(+) cells can be used to reconstruct corneal surfaces. Because neither BM cells nor CD117(+) cells were integrated into the corneal epithelium, we suggest that soluble factors could be responsible for the positive effect of BM cells and CD117(+) cells on corneal wound healing.
Authors with CRIS profile
Fabian Garreis
Lehrstuhl für Funktionelle und Klinische Anatomie
Friedrich Paulsen
Lehrstuhl für Funktionelle und Klinische Anatomie
Involved external institutions
Martin-Luther-Universität Halle-Wittenberg (MLU)
Germany (DE)
Otto-von-Guericke-Universität Magdeburg
Germany (DE)
Fraunhofer-Institut für Angewandte Polymerforschung (IAP) / Fraunhofer Institute for Applied Polymer Research
Germany (DE)
Germany (DE)
Otto-von-Guericke-Universität Magdeburg
Germany (DE)
Fraunhofer-Institut für Angewandte Polymerforschung (IAP) / Fraunhofer Institute for Applied Polymer Research
Germany (DE)
How to cite
APA:
Sel, S., Schilling, U.M., Nass, N., Simm, A., Garreis, F., Knak, M.,... Paulsen, F. (2012). Bone marrow cells and CD117-positive haematopoietic stem cells promote corneal wound healing. Acta Ophthalmologica, 90(5), e367-e373. https://doi.org/10.1111/j.1755-3768.2012.02388.x
MLA:
Sel, Saadettin, et al. "Bone marrow cells and CD117-positive haematopoietic stem cells promote corneal wound healing." Acta Ophthalmologica 90.5 (2012): e367-e373.
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