The influence of 2 kbar pressure on the global and internal dynamics of human hemoglobin observed by quasielastic neutron scattering

Appavou MS, Busch S, Doster W, Gaspar AM, Unruh T (2011)

Publication Status: Published

Publication Type: Journal article

Publication year: 2011

Journal

European Biophysics Journal Springer

Publisher: SPRINGER

Book Volume: 40

Pages Range: 705-714

Journal Issue: 6

DOI: 10.1007/s00249-011-0678-3

Abstract

Pressure is a ubiquitous physical parameter in life and is commonly used in the life sciences to study new protein folding pathways or association-dissociation phenomena. In this paper, an investigation of the influence of pressure on hemoglobin, a multimeric protein, at the picosecond time scale is presented using time-of-flight neutron scattering. The aim is to observe the influence of pressure on the translational diffusion and internal motions of hemoglobin in a concentrated solution and a possible dissociation of the subunits as suggested by Pin et al. (Biochemistry 29:9194, 1990) using fluorescence spectroscopy. A new flat 2 kbar pressure cell made of an aluminum alloy has been used, which allowed the effect of pressure to be studied with minimum background contribution. Within this range of pressure, the effect of this physical parameter on global diffusion can be explained in terms of the change in the water buffer viscosity and an oligomerization of hemoglobin subunits, whereas the internal motions were less affected.

Authors with CRIS profile

Tobias Unruh Professur für Nanomaterialcharakterisierung (Streumethoden)

Involved external institutions

Forschungszentrum Jülich / Research Centre Jülich (FZJ) DE Germany (DE) Technische Universität München (TUM) DE Germany (DE)

How to cite

APA:

Appavou, M.-S., Busch, S., Doster, W., Gaspar, A.M., & Unruh, T. (2011). The influence of 2 kbar pressure on the global and internal dynamics of human hemoglobin observed by quasielastic neutron scattering. European Biophysics Journal, 40(6), 705-714. https://dx.doi.org/10.1007/s00249-011-0678-3

MLA:

Appavou, Marie-Sousai, et al. "The influence of 2 kbar pressure on the global and internal dynamics of human hemoglobin observed by quasielastic neutron scattering." European Biophysics Journal 40.6 (2011): 705-714.

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