Endothelin-1 increases 2-arachidonoyl glycerol (2-AG) production in astrocytes.

Walter L (2003)


Publication Status: Published

Publication Type: Journal article

Publication year: 2003

Journal

Book Volume: 44

Pages Range: 85-90

Journal Issue: 1

DOI: 10.1002/glia.10270

Abstract

Astrocytes play an important role in neuroprotective responses. Recent studies indicate that endothelin-1, a neuropeptide upregulated during brain injury, increases levels of the endocannabinoid anandamide, a lipid with neuroprotective properties, in astrocytes in primary cultures. However, whether this neuropeptide also alters levels of 2-arachidonoyl glycerol (2-AG), the most abundant endocannabinoid in the CNS, in astrocytes remains unknown. In addition, 2-AG levels in astrocytes have never been measured. In this report we use chemical ionization gas chromatography/mass spectrometry to quantify picomole amounts of 2-AG in primary cultures of mouse astrocytes. We also demonstrate that endothelin-1 increases 2-AG production by 5-fold in these cells, a response that requires extracellular calcium and endothelin-1(A) receptor engagement. Immunocytochemistry showed that although cultured mouse neurons and microglia express cannabinoid receptors, cultured astrocytes do not. The data suggest that endothelin-1 modulates 2-AG production in astrocytes and that this endocannabinoid may participate in paracrine signaling toward neurons and microglia.

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How to cite

APA:

Walter, L. (2003). Endothelin-1 increases 2-arachidonoyl glycerol (2-AG) production in astrocytes. Glia, 44(1), 85-90. https://dx.doi.org/10.1002/glia.10270

MLA:

Walter, Lisa. "Endothelin-1 increases 2-arachidonoyl glycerol (2-AG) production in astrocytes." Glia 44.1 (2003): 85-90.

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