Neocellularization and neovascularization of nanosized bioactive glass-coated decellularized trabecular bone scaffolds
Gerhardt LC, Widdows KL, Erol MM, Nandakumar A, Roqan IS, Ansari T, Boccaccini AR (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Publisher: Wiley-Blackwell
Book Volume: 101A
Pages Range: 827-841
DOI: 10.1002/jbm.a.34373
Abstract
In this study, the in vivo recellularization and neovascularization of nanosized bioactive glass (n-BG)-coated decellu-larized trabecular bone scaffolds were studied in a rat model and quantified using stereological analyses. Based on the highest amount of vascular endothelial growth factor (VEGF) secreted by human fibroblasts grown on n-BG coatings (0-1.245 mg/cm 2), decellularized trabecular bone samples (porosity: 43-81%) were coated with n-BG particles. Grown on n-BG particles at a coating density of 0.263 mg/cm2, human fibroblasts produced 4.3 times more VEGF than on uncoated controls. After 8 weeks of implantation in Sprague-Dawley rats, both uncoated and n-BG-coated samples were well infiltrated with newly formed tissue (47-48%) and blood vessels (3-4%). No significant differences were found in cellularization and vascularization between uncoated bone scaffolds and n-BG-coated scaffolds. This finding indicates that the decellularized bone itself may exhibit growth-promoting properties induced by the highly interconnected pore microarchitecture and/or proteins left behind on decellularized scaffolds. Even if we did not find proangiogenic effects in n-BG-coated bone scaffolds, a bioactive coating is considered to be beneficial to impart osteoinductive and osteoconductive properties to decellularized bone. n-BG-coated bone grafts have thus high clinical potential for the regeneration of complex tissue defects given their ability for recellularization and neovascularization. © 2012 Wiley Periodicals, Inc.
Authors with CRIS profile
Involved external institutions
The Griffin Institute - Northwick Park Institute for Medical Research (NPIMR)
United Kingdom (GB)
Imperial College London / The Imperial College of Science, Technology and Medicine
United Kingdom (GB)
Istanbul Technical University / İstanbul Teknik Üniversitesi (İTÜ)
Turkey (TR)
Xeltis BV
Netherlands (NL)
King Abdullah University of Science and Technology (KAUST) / جامعة الملك عبد الله للعلوم و التقنية
Saudi Arabia (SA)
United Kingdom (GB)
Imperial College London / The Imperial College of Science, Technology and Medicine
United Kingdom (GB)
Istanbul Technical University / İstanbul Teknik Üniversitesi (İTÜ)
Turkey (TR)
Xeltis BV
Netherlands (NL)
King Abdullah University of Science and Technology (KAUST) / جامعة الملك عبد الله للعلوم و التقنية
Saudi Arabia (SA)
How to cite
APA:
Gerhardt, L.-C., Widdows, K.L., Erol, M.M., Nandakumar, A., Roqan, I.S., Ansari, T., & Boccaccini, A.R. (2013). Neocellularization and neovascularization of nanosized bioactive glass-coated decellularized trabecular bone scaffolds. Journal of Biomedical Materials Research Part A, 101A, 827-841. https://dx.doi.org/10.1002/jbm.a.34373
MLA:
Gerhardt, L. -C., et al. "Neocellularization and neovascularization of nanosized bioactive glass-coated decellularized trabecular bone scaffolds." Journal of Biomedical Materials Research Part A 101A (2013): 827-841.
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