Journal article


Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana


Publication Details
Author(s): Kost B, Potrykus I, Neuhaus G
Publisher: Springer Verlag (Germany)
Publication year: 1992
Volume: 12
Journal issue: 1
Pages range: 50-54
ISSN: 0721-7714

Abstract

We present protocols for the regeneration of fertile plants from immature zygotic embryos of Arabidopsis thaliana ecotype "Zurich" either directly by multiple shoot formation from the apical region or indirectly by shoot induction from embryo derived calli. The regeneration efficiency by "multiple shooting" depended on the developmental stage of the cultured embryos and ranged from 15% for early heart shaped to 90% for early torpedo shaped and further developed embryos. 85% callus induction was achieved from embryos in the early torpedo shaped or a later stage of development. The efficiency of shoot induction from embryo derived calli varied between 25% and 75% in different experiments.



How to cite
APA: Kost, B., Potrykus, I., & Neuhaus, G. (1992). Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana. Plant Cell Reports, 12(1), 50-54. https://dx.doi.org/10.1007/BF00232423

MLA: Kost, Benedikt, Ingo Potrykus, and Gunther Neuhaus. "Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana." Plant Cell Reports 12.1 (1992): 50-54.

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