Journal article


Rac homologues and compartmentalized phosphatidylinositol 4,5-bisphosphate act in a common pathway to regulate polar pollen tube growth


Publication Details
Author(s): Kost B, Lemichez E, Spielhofer P, Hong Y, Tolias K, Carpenter C, Chua N
Publisher: Rockefeller University Press
Publication year: 1999
Volume: 145
Journal issue: 2
Pages range: 317-330
ISSN: 0021-9525

Abstract

Pollen tube cells elongate based on actin-dependent targeted secretion at the tip, Rho family small GTPases have been implicated in the regulation of related processes in animal and yeast cells. We have functionally characterized Rac type Rho family proteins that are expressed in growing pollen tubes. Expression of dominant negative Rac inhibited pollen tube elongation, whereas expression of constitutive active Pac induced depolarized growth, Pollen tube Rac was found to accumulate at the tip plasma membrane and to physically associate with a phosphatidylinositol monophosphate kinase (PtdIns P-K) activity, Phosphatidylinositol 4, 5-bisphosphate (PtdIns 4, 5-P-2), the product of PtdIns P-Ks, showed a similar intracellular localization as Pac, Expression of the pleckstrin homology (PH)-domain of phospholipase C (PLC)-delta(1), which binds specifically to PtdIns 4, 5-P-2 , inhibited pollen tube elongation. These results indicate that Rac and PtdIns 4, 5-P-2 act in a common pathway to control polar pollen tube growth and provide direct evidence for a function of PtdIns 4, 5-P-2 compartmentalization in the regulation of this process.



How to cite
APA: Kost, B., Lemichez, E., Spielhofer, P., Hong, Y., Tolias, K., Carpenter, C., & Chua, N. (1999). Rac homologues and compartmentalized phosphatidylinositol 4,5-bisphosphate act in a common pathway to regulate polar pollen tube growth. Journal of Cell Biology, 145(2), 317-330. https://dx.doi.org/10.1083/jcb.145.2.317

MLA: Kost, Benedikt, et al. "Rac homologues and compartmentalized phosphatidylinositol 4,5-bisphosphate act in a common pathway to regulate polar pollen tube growth." Journal of Cell Biology 145.2 (1999): 317-330.

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