Journal article


High efficiency transient and stable transformation by optimized DNA microinjection into Nicotiana tabacum protoplasts


Publication Details
Author(s): Kost B, Galli A, Potrykus I, Neuhaus G
Publisher: Oxford University Press (OUP): Policy B - Oxford Open Option B / Oxford University Press (OUP)
Publication year: 1995
Volume: 46
Journal issue: 290
Pages range: 1157-1167
ISSN: 0022-0957

Abstract

Protoplasts were immobilized above a grid pattern and the location of injected cells was recorded by Polaroid photography, The fate of particular targeted cells could be observed, isolation and individual culture of clones derived from injected cells was possible. Following cytoplasmic coinjection of FITC-dextran and 1 mg ml(-1) plasmid DNA on average about 20% of the targeted cells developed into microcalli and roughly 50% of these calli were stably transformed. Transient. expression of the firefly luciferase gene (Luc) was nondestructively analysed 24 h after injection of pAMLuc. Approximately 50% of the injected cells that were alive at this time point expressed the Luc gene transiently. Apparently, stable integration of the injected genes occurred in essentially all transiently expressing cells that developed into clones.



How to cite
APA: Kost, B., Galli, A., Potrykus, I., & Neuhaus, G. (1995). High efficiency transient and stable transformation by optimized DNA microinjection into Nicotiana tabacum protoplasts. Journal of Experimental Botany, 46(290), 1157-1167. https://dx.doi.org/10.1093/jxb/46.9.1157

MLA: Kost, Benedikt, et al. "High efficiency transient and stable transformation by optimized DNA microinjection into Nicotiana tabacum protoplasts." Journal of Experimental Botany 46.290 (1995): 1157-1167.

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