Journal article


NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases


Publication Details
Author(s): Potocky M, Pejchar P, Gutkowska M, Jose Jimenez-Quesada M, Potocka A, De Dios Alche J, Kost B, Zarsky V
Publisher: Elsevier
Publication year: 2012
Volume: 169
Journal issue: 16
Pages range: 1654-1663
ISSN: 0176-1617

Abstract

Reactive oxygen species (ROS) generated by NADPH oxidase (NOX) are crucial for tip growth of pollen tubes. However, the regulation of NOX activity in pollen tubes remains unknown. Using purified plasma membrane fractions from tobacco and olive pollen and tobacco BY-2 cells, we demonstrate that pollen NOX is activated by calcium ions and low abundant signaling phospholipids, such as phosphatidic acid and phosphatidylinositol 4,5-bisphosphate in vitro and in vivo. Our data also suggest possible synergism between Ca2+ and phospholipid-mediated NOX activation in pollen. Rac/Rop small GTPases are also necessary for normal pollen tube growth and have been proposed to regulate ROS production in root hairs. We show here elevated ROS formation in pollen tubes overexpressing wild-type NtRac5 and constitutively active NtRac5, while overexpression of dominant-negative NtRac5 led to a decrease of ROS in pollen tubes. We also show that PA formed by distinct phospholipases D (PLD) is involved in pathways both upstream and downstream of NOX-mediated ROS generation and identify NtPLD delta as a PLD isoform acting in the ROS response pathway. (c) 2012 Elsevier GmbH. All rights reserved.



How to cite
APA: Potocky, M., Pejchar, P., Gutkowska, M., Jose Jimenez-Quesada, M., Potocka, A., De Dios Alche, J.,... Zarsky, V. (2012). NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases. Journal of Plant Physiology, 169(16), 1654-1663. https://dx.doi.org/10.1016/j.jplph.2012.05.014

MLA: Potocky, Martin, et al. "NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases." Journal of Plant Physiology 169.16 (2012): 1654-1663.

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