Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Lu W, Ji K, Kirkham J, Yan Y, Boccaccini AR, Kellett M, Jin Y, Yang XB (2014)


Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2014

Journal

Book Volume: 356

Pages Range: 97-107

Journal Issue: 1

DOI: 10.1007/s00441-013-1770-z

Abstract

Translational research in bone tissue engineering is essential for "bench to bedside" patient benefit. However, the ideal combination of stem cells and biomaterial scaffolds for bone repair/regeneration is still unclear. The aim of this study is to investigate the osteogenic capacity of a combination of poly(DL-lactic acid) (PDLLA) porous foams containing 5 wt% and 40 wt% of Bioglass particles with human adipose-derived stem cells (ADSCs) in vitro and in vivo. Live/dead fluorescent markers, confocal microscopy and scanning electron microscopy showed that PDLLA/Bioglass porous scaffolds supported ADSC attachment, growth and osteogenic differentiation, as confirmed by enhanced alkaline phosphatase (ALP) activity. Higher Bioglass content of the PDLLA foams increased ALP activity compared with the PDLLA only group. Extracellular matrix deposition after 8 weeks in the in vitro cultures was evident by Alcian blue/Sirius red staining. In vivo bone formation was assessed by using scaffold/ADSC constructs in diffusion chambers transplanted intraperitoneally into nude mice and recovered after 8 weeks. Histological and immunohistochemical assays indicated significant new bone formation in the 40 wt% and 5 wt% Bioglass constructs compared with the PDLLA only group. Thus, the combination of a well-developed biodegradable bioactive porous PDLLA/Bioglass composite scaffold with a high-potential stem cell source (human ADSCs) could be a promising approach for bone regeneration in a clinical setting. © 2014 Springer-Verlag Berlin Heidelberg.

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How to cite

APA:

Lu, W., Ji, K., Kirkham, J., Yan, Y., Boccaccini, A.R., Kellett, M.,... Yang, X.B. (2014). Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells. Cell and Tissue Research, 356(1), 97-107. https://doi.org/10.1007/s00441-013-1770-z

MLA:

Lu, Wei, et al. "Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells." Cell and Tissue Research 356.1 (2014): 97-107.

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