Development of an analytical approach for identification and quantification of 5-alpha-androst-16-en-3-one in human milk

Hartmann C, Mayenzet F, Larcinese JP, Haefliger OP, Büttner A, Starkenmann C (2013)


Publication Status: Published

Publication Type: Journal article

Publication year: 2013

Journal

Publisher: Elsevier

Book Volume: 78

Pages Range: 156-160

Journal Issue: 2

DOI: 10.1016/j.steroids.2012.11.005

Abstract

A method was developed for the quantification of 5-alpha-androst-16-en-3-one in human breast milk based on application of a stable isotope dilution assay using 5 alpha-androst-16-en-3-one-6, 6-d(2). The procedure includes extraction of the human milk by hexane with subsequent clean-up of the obtained extract by gel permeation and silica gel column chromatography. The extracted samples were analyzed by gas chromatography-mass spectrometry. Using this method 5-alpha-androst-16-en-3-one could be identified and for the first time quantified in a concentration range of 26-155 ng/kg in human milk. (C) 2012 Elsevier Inc. All rights reserved.

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APA:

Hartmann, C., Mayenzet, F., Larcinese, J.-P., Haefliger, O.P., Büttner, A., & Starkenmann, C. (2013). Development of an analytical approach for identification and quantification of 5-alpha-androst-16-en-3-one in human milk. Steroids, 78(2), 156-160. https://dx.doi.org/10.1016/j.steroids.2012.11.005

MLA:

Hartmann, Constanze, et al. "Development of an analytical approach for identification and quantification of 5-alpha-androst-16-en-3-one in human milk." Steroids 78.2 (2013): 156-160.

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