Chondrogenesis of human bone marrow mesenchymal stromal cells in highly porous alginate-foams supplemented with chondroitin sulfate

Huang Z, Nooeaid P, Kohl B, Roether J, Schubert DW, Meier C, Boccaccini AR, Godkin O, Ertel W, Arens S, Schulze-Tanzil G (2015)

Publication Status: Published

Publication Type: Journal article, Review article

Publication year: 2015

Journal

Publisher: Elsevier

Book Volume: 50

Pages Range: 160-172

DOI: 10.1016/j.msec.2015.01.082

Abstract

To overcome the limited intrinsic cartilage repair, autologous chondrocyte or bone-marrow-derived mesenchymal stromal cell (BM-MSC) was implanted into cartilage defects. For this purpose suitable biocompatible scaffolds are needed to provide cell retention, chondrogenesis and initial mechanical stability. The present study should indicate whether a recently developed highly porous alginate (Alg) foam scaffold supplemented with chondroitin sulfate (CS) allows the attachment, survival and chondrogenesis of BM-MSCs and articular chondrocytes. The foams were prepared using a freeze-drying method; some of them were supplemented with CS and subsequently characterized for porosity, biodegradation and mechanical profile. BM-MSCs were cultured for 1-2 weeks on the scaffold either under chondrogenic or maintenance conditions. Cell vitality assays, histology, glycosaminoglycan (sGAG) assay, and type II and I collagen immunolabelings were performed to monitor cell growth and extracellular matrix (ECM) synthesis in the scaffolds. Scaffolds had a high porosity similar to 93-95% with a mean pore sizes of 237 +/- 48 mu m (Alg) and 197 +/- 61 mu m (Alg/CS). Incorporation of CS increased mechanical strength of the foams providing gradually CS release over 7 days. Most of the cells survived in the scaffolds. BM-MSCs and articular chondrocytes formed rounded clusters within the scaffold pores. The BM-MSCs, irrespective of whether cultured under non/chondrogenic conditions and chondrocytes produced an ECM containing sGAGs, and types II and I collagen. Total collagen and sGAG contents were higher in differentiated BM-MSC cultures supplemented with CS than in CS-free foams after 14 days. The cell cluster formation induced by the scaffolds might stimulate chondrogenesis via initial intense cell-cell contacts. (C) 2015 Elsevier B.V. All rights reserved.

Authors with CRIS profile

Involved external institutions

Charité - Universitätsmedizin Berlin Germany (DE)

How to cite

APA:

Huang, Z., Nooeaid, P., Kohl, B., Roether, J., Schubert, D.W., Meier, C.,... Schulze-Tanzil, G. (2015). Chondrogenesis of human bone marrow mesenchymal stromal cells in highly porous alginate-foams supplemented with chondroitin sulfate. Materials Science and Engineering C, 50, 160-172. https://dx.doi.org/10.1016/j.msec.2015.01.082

MLA:

Huang, Zhao, et al. "Chondrogenesis of human bone marrow mesenchymal stromal cells in highly porous alginate-foams supplemented with chondroitin sulfate." Materials Science and Engineering C 50 (2015): 160-172.

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