Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic
Stancic A, Otasevic V, Jankovic A, Vucetic M, Ivanovic-Burmazovic I, Filipovic M, Korac A, Markelic M, Velickovic K, Golic I, Buzadzic B, Korac B (2013)
Publication Type: Journal article, Original article
Publication year: 2013
Journal
Original Authors: Stančić A., Otašević V., Janković A., Vučetić M., Ivanović-Burmazović I., Filipović M., Korać A., Markelić M., Veličković K., Golić I., Buzadžić B., Korać B.
Publisher: Elsevier
Book Volume: 99
Pages Range: 27-33
Journal Issue: null
DOI: 10.1016/j.brainresbull.2013.09.009
Abstract
Hippocampal structural changes associated with diabetes-related cognitive impairments are well described, but their molecular background remained vague. We examined whether/how diabetes alters molecular basis of energy metabolism in hippocampus readily after diabetes onset, with special emphasis on its redox-sensitivity.To induce diabetes, adult Mill Hill hybrid hooded rats received a single alloxan dose (120mg/kg). Both non-diabetic and diabetic groups were further divided in two subgroups receiving (i) or not (ii) superoxide dismutase (SOD) mimic, [Mn(II)(pyane)Cl] for 7 days, i.p. Treatment of the diabetic animals started after blood glucose level ≥12mM.Diabetes decreased protein levels of oxidative phosphorylation components: complex III and ATP synthase. In contrast, protein amounts of glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase, and hypoxia-inducible factor-1α - the key regulator of energy metabolism in stress conditions, were higher in diabetic animals. Treatment with SOD mimic restored/increased the levels of oxidative phosphorylation components and returned hypoxia-inducible factor-1α to control level, while diabetes-induced up-regulation of glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, was additionally stimulated.To conclude, our results provide insight into the earliest molecular changes of energy-producing pathways in diabetes that may account for structural/functional disturbance of hippocampus, seen during disease progression. Also, data suggest [Mn(II)(pyane)Cl] as potential therapeutic agent in cutting-edge approaches to threat this widespread metabolic disorder. © 2013 Elsevier Inc.
Authors with CRIS profile
Ivana Ivanovic-Burmazovic
Lehrstuhl für Bioanorganische Chemie
Milos Filipovic
Lehrstuhl für Bioanorganische Chemie
Involved external institutions
Serbia (RS)How to cite
APA:
Stancic, A., Otasevic, V., Jankovic, A., Vucetic, M., Ivanovic-Burmazovic, I., Filipovic, M.,... Korac, B. (2013). Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic. Brain Research Bulletin, 99(null), 27-33. https://doi.org/10.1016/j.brainresbull.2013.09.009
MLA:
Stancic, Ana, et al. "Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic." Brain Research Bulletin 99.null (2013): 27-33.
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