In vivo imaging of the rodent eye with swept source/Fourier domain OCT

Liu JJ, Grulkowski I, Kraus M, Potsaid B, Lu CD, Baumann B, Duker JS, Hornegger J, Fujimoto JG (2013)

Publication Type: Journal article, Original article

Publication year: 2013

Journal

Biomedical Optics Express Optical Society of America

Original Authors: Liu J., Grulkowski I., Kraus M., Potsaid B., Lu C., Baumann B., Duker J., Hornegger J., Fujimoto J.

Publisher: Optical Society of America

Book Volume: 4

Pages Range: 351-363

Journal Issue: 2

DOI: 10.1364/BOE.4.000351

Abstract

Swept source/Fourier domain OCT is demonstrated for in vivo imaging of the rodent eye. Using commercial swept laser technology, we developed a prototype OCT imaging system for small animal ocular imaging operating in the 1050 nm wavelength range at an axial scan rate of 100 kHz with ~6 μm axial resolution. The high imaging speed enables volumetric imaging with high axial scan densities, measuring high flow velocities in vessels, and repeated volumetric imaging over time. The 1050 nm wavelength light provides increased penetration into tissue compared to standard commercial OCT systems at 850 nm. The long imaging range enables multiple operating modes for imaging the retina, posterior eye, as well as anterior eye and full eye length. A registration algorithm using orthogonally scanned OCT volumetric data sets which can correct motion on a per A-scan basis is applied to compensate motion and merge motion corrected volumetric data for enhanced OCT image quality. Ultrahigh speed swept source OCT is a promising technique for imaging the rodent eye, proving comprehensive information on the cornea, anterior segment, lens, vitreous, posterior segment, retina and choroid. © 2013 Optical Society of America.

Authors with CRIS profile

Martin Kraus Lehrstuhl für Informatik 14 (Bild- und Sprachverarbeitung) Joachim Hornegger Lehrstuhl für Informatik 14 (Bild- und Sprachverarbeitung)

Involved external institutions

Massachusetts Institute of Technology (MIT) US United States (USA) (US) Tufts University US United States (USA) (US)

How to cite

APA:

Liu, J.J., Grulkowski, I., Kraus, M., Potsaid, B., Lu, C.D., Baumann, B.,... Fujimoto, J.G. (2013). In vivo imaging of the rodent eye with swept source/Fourier domain OCT. Biomedical Optics Express, 4(2), 351-363. https://dx.doi.org/10.1364/BOE.4.000351

MLA:

Liu, Jonathan J., et al. "In vivo imaging of the rodent eye with swept source/Fourier domain OCT." Biomedical Optics Express 4.2 (2013): 351-363.

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