Neutron spectroscopy on protein solutions employing backscattering with an increased energy range

Beck C, Appel M, Grimaldo M, Roosen-Runge F, Zhang F, Frick B, Schreiber F, Seydel T (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Physica B-Condensed Matter Elsevier

Book Volume: 562

Pages Range: 31-35

DOI: 10.1016/j.physb.2018.11.058

Abstract

Novel cold neutron backscattering spectrometers contribute substantially to the understanding of the diffusive dynamics of proteins in dense aqueous suspensions. Such suspensions are fundamentally interesting for instance in terms of the so-called macromolecular crowding, protein cluster formation, gelation, and self-assembly. Notably, backscattering spectrometers with the highest flux can simultaneously access the center-of-mass diffusion of the proteins and the superimposed internal molecular diffusive motions. The nearly complete absence of protein-protein collisions on the accessible nanosecond observation time scale even in dense protein suspensions implies that neutron backscattering accesses the so-called short-time limit for the center-of-mass diffusion. This limit is particularly interesting in terms of a theoretical understanding by concepts from colloid physics. Here we briefly review recent progress in studying protein dynamics achieved with the latest generation of backscattering spectrometers. We illustrate this progress by the first data from a protein solution using the backscattering-and-time-of-flight option BATS on IN16B at the ILL and we outline future perspectives.

Authors with CRIS profile

Markus Appel Lehrstuhl für Biophysik

Additional Organisation(s)

Zentrum für Medizinische Physik und Technik

Involved external institutions

Institute Laue-Langevin (ILL) FR France (FR) Eberhard Karls Universität Tübingen DE Germany (DE) Lund University / Lunds universitet SE Sweden (SE)

How to cite

APA:

Beck, C., Appel, M., Grimaldo, M., Roosen-Runge, F., Zhang, F., Frick, B.,... Seydel, T. (2019). Neutron spectroscopy on protein solutions employing backscattering with an increased energy range. Physica B-Condensed Matter, 562, 31-35. https://dx.doi.org/10.1016/j.physb.2018.11.058

MLA:

Beck, Christian, et al. "Neutron spectroscopy on protein solutions employing backscattering with an increased energy range." Physica B-Condensed Matter 562 (2019): 31-35.

BibTeX: Download