Traction Force Microscopy in 3-Dimensional Extracellular Matrix Networks.
Cóndor M, Steinwachs J, Mark C, Garcia-Aznar JM, Fabry B (2017)
Publication Status: Published
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 75
Pages Range: 10.22.1-10.22.20
URI: https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/cpcb.24
DOI: 10.1002/cpcb.24
Open Access Link: https://www.sciencedirect.com/science/article/pii/S0142961210008768
Abstract
Cell migration through a three-dimensional (3-D) matrix depends strongly on the ability of cells to generate traction forces. To overcome the steric hindrance of the matrix, cells need to generate sufficiently high traction forces but also need to distribute these forces spatially in a migration-promoting way. This unit describes a protocol to measure spatial maps of cell traction forces in 3-D biopolymer networks such as collagen, fibrin, or Matrigel. Traction forces are computed from the relationship between measured force-induced matrix deformations surrounding the cell and the known mechanical properties of the matrix. The method does not rely on knowledge of the cell surface coordinates and takes nonlinear mechanical properties of the matrix into account. © 2017 by John Wiley & Sons, Inc.
Authors with CRIS profile
Julian Steinwachs
Lehrstuhl für Biophysik
Christoph Mark
Lehrstuhl für Biophysik
Ben Fabry
Lehrstuhl für Biophysik
Involved external institutions
Spain (ES)How to cite
APA:
Cóndor, M., Steinwachs, J., Mark, C., Garcia-Aznar, J.M., & Fabry, B. (2017). Traction Force Microscopy in 3-Dimensional Extracellular Matrix Networks. Current protocols in cell biology, 75, 10.22.1-10.22.20. https://dx.doi.org/10.1002/cpcb.24
MLA:
Cóndor, Mar, et al. "Traction Force Microscopy in 3-Dimensional Extracellular Matrix Networks." Current protocols in cell biology 75 (2017): 10.22.1-10.22.20.
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