Quantitative Ratiometric Ca(2+) Imaging to Assess Cell Viability.

Friedrich O, Head S (2017)


Publication Language: English

Publication Status: Published

Publication Type: Journal article

Publication year: 2017

Journal

Book Volume: 1601

Pages Range: 171-193

DOI: 10.1007/978-1-4939-6960-9_14

Abstract

Viability of cells is strongly related to their Ca(2+) homeostasis. Ca(2+) signal fluctuations can be on a slow time scale, e.g., in non-excitable cells, but also in the range of tens of milliseconds for excitable cells, such as nerve and muscle. Muscle fibers respond to electrical stimulation with Ca(2+) transients that exceed their resting basal level about 100 times. Fluorescent Ca(2+) dyes have become an indispensable means to monitor Ca(2+) fluctuations in living cells online. Fluorescence intensity of such "environmental dyes" relies on a buffer-ligand interaction which is not only governed by laws of mass action but also by binding and unbinding kinetics that have to be considered for proper Ca(2+) kinetics and amplitude validation. The concept of Ca(2+) dyes including the different approaches using ratiometric and non-ratiometric dyes, the way to correctly choose dyes according to their low-/high-affinity properties and kinetics as well as staining techniques, and in situ calibration are reviewed and explained. We provide detailed protocols to apply ratiometric Fura-2 imaging of resting Ca(2+) and Ca(2+) fluctuations during field-stimulation in single isolated skeletal muscle cells and how to translate fluorescence intensities into absolute Ca(2+) concentrations using appropriate calibration techniques.

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APA:

Friedrich, O., & Head, S. (2017). Quantitative Ratiometric Ca(2+) Imaging to Assess Cell Viability. Methods in Molecular Biology, 1601, 171-193. https://dx.doi.org/10.1007/978-1-4939-6960-9_14

MLA:

Friedrich, Oliver, and Stewart Head. "Quantitative Ratiometric Ca(2+) Imaging to Assess Cell Viability." Methods in Molecular Biology 1601 (2017): 171-193.

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