lThe stress granule component G3BP is a novel interaction partner for the nuclear shuttle proteins of the nanovirus pea necrotic yellow dwarf virus and geminivirus abutilon mosaic virus

Krapp S, Greiner E, Amin B, Sonnewald U, Krenz B (2017)

Publication Status: Published

Publication Type: Journal article

Publication year: 2017

Journal

Publisher: ELSEVIER SCIENCE BV

Book Volume: 227

Pages Range: 6-14

DOI: 10.1016/j.virusres.2016.09.021

Abstract

Stress granules (SGs) are structures within cells that regulate gene expression during stress response, e.g. viral infection. In mammalian cells assembly of SGs is dependent on the Ras-GAP SH3-domain-binding protein (G3BP). The C-terminal domain of the viral nonstructural protein 3 (nsP3) of Semliki Forest virus (SFV) forms a complex with mammalian G3BP and sequesters it into viral RNA replication complexes in a manner that inhibits the formation of SGs. The binding-domain of nsP3 to HsG3BP was mapped to two tandem 'FGDF' repeat motifs close to the C-terminus of the viral proteins. It was speculated that plant viruses employ a similar strategy to inhibit SG function. This study identifies an Arabidopsis thaliana NTF2-RRM domain-containing protein as a G3BP-like protein (AtG3BP), which localizes to plant SGs. Moreover, the nuclear shuttle protein (NSP) of the begomovirus abutilon mosaic virus (AbMV), which harbors a 'FVSF'-motif at its C-terminal end, interacts with the AtG3BP-like protein, as does the TNGSF'motif containing NSP of pea necrotic yellow dwarf virus (PNYDV), a member of the Nanoviridae family. We therefore propose that SG formation upon stress is conserved between mammalian and plant cells and that plant viruses may follow a similar strategy to inhibit plant SG function as it has been shown for their mammalian counterparts. (C) 2016 Elsevier B.V. All rights reserved.

Authors with CRIS profile

Involved external institutions

University of Pittsburgh United States (USA) (US)

How to cite

APA:

Krapp, S., Greiner, E., Amin, B., Sonnewald, U., & Krenz, B. (2017). lThe stress granule component G3BP is a novel interaction partner for the nuclear shuttle proteins of the nanovirus pea necrotic yellow dwarf virus and geminivirus abutilon mosaic virus. Virus Research, 227, 6-14. https://doi.org/10.1016/j.virusres.2016.09.021

MLA:

Krapp, Susanna, et al. "lThe stress granule component G3BP is a novel interaction partner for the nuclear shuttle proteins of the nanovirus pea necrotic yellow dwarf virus and geminivirus abutilon mosaic virus." Virus Research 227 (2017): 6-14.

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