Journal article


Influence of different irradiation protocols on vascularization and bone formation parameters in rat femora.


Publication Details
Author(s): Rottensteiner-Brandl U, Distel L, Stumpf M, Fey T, Köhn K, Bertram U, Lingens LF, Greil P, Horch R, Arkudas A
Publication year: 2017
ISSN: 1937-3392

Abstract

INTRODUCTION Aim of the present study was the establishment of an efficient and reproducible model for irradiation of rat femora as a model for impaired osteogenesis and angiogenesis. MATERIALS AND METHODS Four different irradiation protocols were compared: single irradiation of the left femur with 20 Gy and explantation after 4 or 8 weeks (group A, B); three irradiation fractions at 3-4 days intervals with 10 Gy and explantation after 4 or 8 weeks (group C, D). The contralateral, unirradiated femur served as control. Evaluation included histology, microcomputertomography (μCT) and real time PCR.\nRESULTS\nHistology showed a pronounced increase of vacuoles in bone marrow after irradiation, especially after 4 weeks (group A and C), demonstrating bone marrow edema and fatty degeneration. Irradiation provoked a decrease of total cell numbers in cortical bone and of Hif1α positive cells in bone marrow. The expression of several markers (osteocalcin, RUNX2, TGFβ1, TNFα, VEGF-A, Hif1α) was decreased in group A after irradiation. This might suggest a decreased metabolism after irradiation. A significant decrease in small-sized vessels was seen in μCT evaluation in group A and D. CONCLUSIONS Single irradiation with 20 Gy had the most severe and reproducible impact on osteogenesis and angiogenesis after 4 weeks while being well tolerated by all animals, thus making it an excellent model for evaluation of bone healing and vascularization in irradiated tissue.



How to cite
APA: Rottensteiner-Brandl, U., Distel, L., Stumpf, M., Fey, T., Köhn, K., Bertram, U.,... Arkudas, A. (2017). Influence of different irradiation protocols on vascularization and bone formation parameters in rat femora. Tissue Engineering - Part C: Methods. https://dx.doi.org/10.1089/ten.TEC.2017.0170

MLA: Rottensteiner-Brandl, Ulrike, et al. "Influence of different irradiation protocols on vascularization and bone formation parameters in rat femora." Tissue Engineering - Part C: Methods (2017).

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