Oxalate-induced chronic kidney disease with its uremic and cardiovascular complications in C57BL/6 mice

Mulay SR, Eberhard JN, Pfann V, Marschner JA, Darisipudi MN, Daniel C, Romoli S, Desai J, Grigorescu M, Kumar SV, Rathkolb B, Wolf E, De Angelis MH, Bäuerle T, Dietel B, Wagner CA, Amann KU, Eckardt KU, Aronson PS, Anders HJ, Knauf F (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Book Volume: 310

Pages Range: F785-F795

Journal Issue: 8

DOI: 10.1152/ajprenal.00488.2015

Abstract

Chronic kidney disease (CKD) research is limited by the lack of convenient inducible models mimicking human CKD and its complications in experimental animals. We demonstrate that a soluble oxalate-rich diet induces stable stages of CKD in male and female C57BL/6 mice. Renal histology is characterized by tubular damage, remnant atubular glomeruli, interstitial inflammation, and fibrosis, with the extent of tissue involvement depending on the duration of oxalate feeding. Expression profiling of markers and magnetic resonance imaging findings established to reflect inflammation and fibrosis parallel the histological changes. Within 3 wk, the mice reproducibly develop normochromic anemia, metabolic acidosis, hyperkalemia, FGF23 activation, hyperphosphatemia, and hyperparathyroidism. In addition, the model is characterized by profound arterial hypertension as well as cardiac fibrosis that persist following the switch to a control diet. Together, this new model of inducible CKD overcomes a number of previous experimental limitations and should serve useful in research related to CKD and its complications.

Authors with CRIS profile

Involved external institutions

Klinikum der Universität München (Großhadern und Innenstadt) Germany (DE) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich Germany (DE) Ludwig-Maximilians-Universität (LMU) Germany (DE) University of Zurich / Universität Zürich (UZH) Switzerland (CH)

How to cite

APA:

Mulay, S.R., Eberhard, J.N., Pfann, V., Marschner, J.A., Darisipudi, M.N., Daniel, C.,... Knauf, F. (2016). Oxalate-induced chronic kidney disease with its uremic and cardiovascular complications in C57BL/6 mice. American Journal of Physiology-Renal Physiology, 310(8), F785-F795. https://doi.org/10.1152/ajprenal.00488.2015

MLA:

Mulay, Shrikant R., et al. "Oxalate-induced chronic kidney disease with its uremic and cardiovascular complications in C57BL/6 mice." American Journal of Physiology-Renal Physiology 310.8 (2016): F785-F795.

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