Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy

Monzel C, Schmidt D, Kleusch C, Kirchenbuechler D, Seifert U, Smith AS, Sengupta K, Merkel R (2015)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2015

Journal

Nature Communications Nature Publishing Group: Nature Communications

Publisher: NATURE PUBLISHING GROUP

Book Volume: 6

Article Number: 8162

URI: http://www.nature.com/articles/ncomms9162

DOI: 10.1038/ncomms9162

Open Access Link: http://www.nature.com/articles/ncomms9162

Abstract

Stochastic displacements or fluctuations of biological membranes are increasingly recognized as an important aspect of many physiological processes, but hitherto their precise quantification in living cells was limited due to a lack of tools to accurately record them. Here we introduce a novel technique-dynamic optical displacement spectroscopy (DODS), to measure stochastic displacements of membranes with unprecedented combined spatio-temporal resolution of 20 nm and 10 mu s. The technique was validated by measuring bending fluctuations of model membranes. DODS was then used to explore the fluctuations in human red blood cells, which showed an ATP-induced enhancement of non-Gaussian behaviour. Plasma membrane fluctuations of human macrophages were quantified to this accuracy for the first time. Stimulation with a cytokine enhanced non-Gaussian contributions to these fluctuations. Simplicity of implementation, and high accuracy make DODS a promising tool for comprehensive understanding of stochastic membrane processes.

Authors with CRIS profile

Daniel Schmidt Physics Underlying Life Science Ana-Suncana Smith Physics Underlying Life Science

Additional Organisation(s)

Exzellenz-Cluster Engineering of Advanced Materials

Related research project(s)

Biological Membranes in Action: A Unified Approach to Complexation, Scaffolding and Active Transport (MEMBRANESACT) Oct. 1, 2013 - Sept. 30, 2019

Involved external institutions

Forschungszentrum Jülich / Research Centre Jülich (FZJ) DE Germany (DE) Universität Stuttgart DE Germany (DE) Aix-Marseille University / Aix-Marseille Université FR France (FR)

How to cite

APA:

Monzel, C., Schmidt, D., Kleusch, C., Kirchenbuechler, D., Seifert, U., Smith, A.-S.,... Merkel, R. (2015). Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy. Nature Communications, 6. https://dx.doi.org/10.1038/ncomms9162

MLA:

Monzel, Cornelia, et al. "Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy." Nature Communications 6 (2015).

BibTeX: Download