Argonaute Family Protein Expression in Normal Tissue and Cancer Entities

Voeller D, Linck L, Bruckmann A, Hauptmann J, Deutzmann R, Meister G, Boßerhoff AK (2016)

Publication Type: Journal article

Publication year: 2016

Journal

PLoS ONE Public Library of Science

Book Volume: 11

Pages Range: e0161165

Journal Issue: 8

DOI: 10.1371/journal.pone.0161165

Abstract

The members of the Argonaute (AGO) protein family are key players in miRNA-guided gene silencing. They enable the interaction between small RNAs and their respective target mRNA(s) and support the catalytic destruction of the gene transcript or recruit additional proteins for downstream gene silencing. The human AGO family consists of four AGO proteins (AGO1-AGO4), but only AGO2 harbors nuclease activity. In this study, we characterized the expression of the four AGO proteins in cancer cell lines and normal tissues with a new mass spectrometry approach called AGO-APP (AGO Affinity Purification by Peptides). In all analyzed normal tissues, AGO1 and AGO2 were most prominent, but marked tissue-specific differences were identified. Furthermore, considerable changes during development were observed by comparing fetal and adult tissues. We also identified decreased overall AGO expression in melanoma derived cell lines compared to other tumor cell lines and normal tissues, with the largest differences in AGO2 expression. The experiments described in this study suggest that reduced amounts of AGO proteins, as key players in miRNA processing, have impact on several cellular processes. Deregulated miRNA expression has been attributed to chromosomal aberrations, promoter regulation and it is known to have a major impact on tumor development and progression. Our findings will further increase our basic understanding of the molecular basis of miRNA processing and its relevance for disease.

Authors with CRIS profile

Lisa Linck-Paulus Lehrstuhl für Biochemie und Molekulare Medizin Anja Katrin Boßerhoff Lehrstuhl für Biochemie und Molekulare Medizin

Involved external institutions

Universität Regensburg DE Germany (DE)

How to cite

APA:

Voeller, D., Linck, L., Bruckmann, A., Hauptmann, J., Deutzmann, R., Meister, G., & Boßerhoff, A.K. (2016). Argonaute Family Protein Expression in Normal Tissue and Cancer Entities. PLoS ONE, 11(8), e0161165. https://dx.doi.org/10.1371/journal.pone.0161165

MLA:

Voeller, Daniel, et al. "Argonaute Family Protein Expression in Normal Tissue and Cancer Entities." PLoS ONE 11.8 (2016): e0161165.

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