ß-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells.
Prasch C, Ott K, Bauer H, Ache P, Hedrich R, Sonnewald U (2015)
Publication Status: Published
Publication Type: Journal article
Publication year: 2015
Journal
Publisher: Oxford University Press (OUP): Policy B - Oxford Open Option B / Oxford University Press (OUP)
Book Volume: 66
Pages Range: 6059-6067
Journal Issue: 19
DOI: 10.1093/jxb/erv323
Abstract
bam1 mutant plants impaired in stomatal starch degradation showed an improved drought tolerance associated with a down-regulation of guard cell-specific gene expression involved in water uptake and cell expansion.In plants, drought stress is a major growth limiting factor causing cell water loss through open stomata. In this study, guard cell-specific transcripts from drought-stressedArabidopsis plants were analysed and a down-regulation of beta-amylase 1 (BAM1) was found. In previous studies, BAM1 was shown to be involved in stomatal starch degradation under ambient conditions. Impaired starch breakdown ofbam1 mutant plants was accompanied by decreased stomatal opening. Here, it is shown that drought tolerance ofbam1 mutant plants is improved as compared with wild-type controls. Microarray analysis of stomata-specific transcripts frombam1 mutant plants revealed a significant down-regulation of genes encoding aquaporins, auxin- and ethylene-responsive factors, and cell-wall modifying enzymes. This expression pattern suggests that reduced water uptake and limited cell wall extension are associated with the closed state of stomata ofbam1 mutant plants. Together these data suggest that regulation of stomata-specific starch turnover is important for adapting stomata opening to environmental needs and its breeding manipulation may result in drought tolerant crop plants.
Authors with CRIS profile
Christian Prasch
Lehrstuhl für Biochemie
Kirsten Ott
Lehrstuhl für Biochemie
Uwe Sonnewald
Lehrstuhl für Biochemie
Involved external institutions
Germany (DE)How to cite
APA:
Prasch, C., Ott, K., Bauer, H., Ache, P., Hedrich, R., & Sonnewald, U. (2015). ß-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells. Journal of Experimental Botany, 66(19), 6059-6067. https://doi.org/10.1093/jxb/erv323
MLA:
Prasch, Christian, et al. "ß-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells." Journal of Experimental Botany 66.19 (2015): 6059-6067.
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