Surface functionalization allowing repetitive use of optical sensors for real-time detection of antibody-bacteria interaction.

Kutscher M, Rosenberger M, Schmauß B, Meinel L, Lorenz U, Ohlsen K, Hellmann R, Germershaus O (2016)

Publication Language: English

Publication Status: Published

Publication Type: Journal article

Publication year: 2016

Journal

Journal of Biophotonics Wiley-VCH Verlag

Book Volume: 9

Pages Range: 730-7

Journal Issue: 7

DOI: 10.1002/jbio.201500178

Abstract

In this study, sensor surface functionalization allowing the repetitive use of a sensing device was evaluated for antibody-based detection of living bacteria using an optical planar Bragg grating sensor. To achieve regenerable immobilization of bacteria specific antibodies, the heterobifunctional cross-linker N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) was linked to an aminosilanized sensor surface and subsequently reduced to expose sulfhydryl groups enabling the covalent conjugation of SPDP-activated antibodies via disulfide bonds. The immobilization of a capture antibody specific for Staphylococcus aureus on the sensor surface as well as specific binding of S. aureus could be monitored, highlighting the applicability of optical sensors for the specific detection of large biological structures. Reusability of bacteria saturated sensors was successfully demonstrated by cleaving the antibody along with bound bacteria through reduction of disulfide bonds and subsequent re-functionalization with activated antibody, resulting in comparable sensitivity towards S. aureus.

Authors with CRIS profile

Bernhard Schmauß Professur für Hochfrequenztechnik mit dem Schwerpunkt Optische Hochfrequenztechnik und Photonik

Involved external institutions

Julius-Maximilians-Universität Würzburg DE Germany (DE) Universitätsklinikum Würzburg DE Germany (DE) Hochschule Aschaffenburg DE Germany (DE)

How to cite

APA:

Kutscher, M., Rosenberger, M., Schmauß, B., Meinel, L., Lorenz, U., Ohlsen, K.,... Germershaus, O. (2016). Surface functionalization allowing repetitive use of optical sensors for real-time detection of antibody-bacteria interaction. Journal of Biophotonics, 9(7), 730-7. https://doi.org/10.1002/jbio.201500178

MLA:

Kutscher, Marika, et al. "Surface functionalization allowing repetitive use of optical sensors for real-time detection of antibody-bacteria interaction." Journal of Biophotonics 9.7 (2016): 730-7.

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