Effect of ion release from Cu-doped 45S5 Bioglass® on 3D endothelial cell morphogenesis

Staehli C, James-Bhasin M, Hoppe A, Boccaccini AR, Nazhat SN (2015)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2015

Journal

Acta Biomaterialia Elsevier

Publisher: Elsevier

Book Volume: 19

Pages Range: 15-22

DOI: 10.1016/j.actbio.2015.03.009

Abstract

Both silicate-based bioactive glasses and copper ions have demonstrated angiogenic activity and therefore represent promising bioinorganic agents for the promotion of vascularization in tissue-engineered scaffolds. This study examined the effect of ionic release products from 45S5 Bioglass® doped with 0 and 2.5 wt.% CuO (BG and Cu-BG respectively) on the formation of capillary-like networks by SVEC4-10 endothelial cells (ECs) seeded in a three-dimensional (3D) type I collagen matrix. Copper and silicon release following 24 h dissolution increased non-proportionally with Cu-BG concentration in cell culture medium, while calcium levels were decreased below the initial medium concentration. EC network length, connectivity, branching, quantified by means of a 3D morphometric image analysis method, as well as proliferation and metabolic activity were reduced in a dose-dependent fashion by BG and Cu-BG ionic release products. This reduction was less prominent for BG compared to an equivalent concentration of Cu-BG, which was attributed to a lower extent of silicon release and calcium consumption. Moreover, a CuCl2 dose equivalent to the highest concentration of Cu-BG exhibited no effect on ECs. In conclusion, while the previously reported pro-angiogenic activity of both Bioglass® and copper may not be reflected in a direct response of ECs, this study provides a maximum glass concentration for non-harmful angiogenic stimulation to be examined in future work.

Authors with CRIS profile

Alexander Hoppe Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

McGill University CA Canada (CA)

How to cite

APA:

Staehli, C., James-Bhasin, M., Hoppe, A., Boccaccini, A.R., & Nazhat, S.N. (2015). Effect of ion release from Cu-doped 45S5 Bioglass® on 3D endothelial cell morphogenesis. Acta Biomaterialia, 19, 15-22. https://dx.doi.org/10.1016/j.actbio.2015.03.009

MLA:

Staehli, Christoph, et al. "Effect of ion release from Cu-doped 45S5 Bioglass® on 3D endothelial cell morphogenesis." Acta Biomaterialia 19 (2015): 15-22.

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