Quantification of reactive carbonyl compounds in icodextrin-based peritoneal dialysis fluids by combined UHPLC-DAD and -MS/MS detection

Gensberger-Reigl S, Huppert J, Pischetsrieder M (2016)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2016

Journal

Journal of Pharmaceutical and Biomedical Analysis Elsevier

Publisher: Elsevier

Book Volume: 118

Pages Range: 132-138

DOI: 10.1016/j.jpba.2015.10.022

Abstract

During heat sterilization of peritoneal dialysis (PD) fluids, the glucose component is partially degraded. The formed glucose degradation products impair biocompatibility and limit the long-term application of PD fluids. As an alternative to glucose, icodextrin, a polyglucose, is used as osmotic agent in PD fluids. After targeted screening for reactive carbonyl compounds, NMR- and MS-analyses very recently revealed 4-deoxyglucosone (4-DG), 3-deoxyglucosone (3-DG), 3-deoxygalactosone (3-DGal), 3,4-dideoxypentosone (3,4-DDPS), and 5-hydroxymethylfurfural (5-HMF) as main polyglucose degradation products (pGDPs) in icodextrin-based PD fluids. Now, the present study established and validated a UHPLC method with DAD as well as a UHPLC-MS/MS method for the first-time quantification of those five major pGDPs in commercial icodextrin PD fluids after derivatization with o-phenylenediamine. Thus, 4-DG was identified to be the main degradation product (in concentrations up to 20 mu M). In contrast to the values measured in glucose-based products, the concentration of 3-DGal (<= 16 mu M) was higher than the concentration of 3-DG (<= 7 mu M) indicating different reaction pathways starting from polyglucose compared to glucose. The compounds 3,4-DDPS and 5-HMF were present in minor quantities (<= 0.3 mu M each). (c) 2015 Elsevier B.V. All rights reserved.

Authors with CRIS profile

Sabrina Gensberger-Reigl Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl) Monika Pischetsrieder Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)

Additional Organisation(s)

Emil-Fischer-Zentrum (Emil Fischer Center)

Involved external institutions

Fresenius Medical Care Deutschland GmbH DE Germany (DE)

How to cite

APA:

Gensberger-Reigl, S., Huppert, J., & Pischetsrieder, M. (2016). Quantification of reactive carbonyl compounds in icodextrin-based peritoneal dialysis fluids by combined UHPLC-DAD and -MS/MS detection. Journal of Pharmaceutical and Biomedical Analysis, 118, 132-138. https://dx.doi.org/10.1016/j.jpba.2015.10.022

MLA:

Gensberger-Reigl, Sabrina, Jochen Huppert, and Monika Pischetsrieder. "Quantification of reactive carbonyl compounds in icodextrin-based peritoneal dialysis fluids by combined UHPLC-DAD and -MS/MS detection." Journal of Pharmaceutical and Biomedical Analysis 118 (2016): 132-138.

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