Journal article


Qualitative profiling of polyglucose degradation products in peritoneal dialysis fluids


Publication Details
Author(s): Gensberger S, Knabner C, Waibel R, Huppert J, Pischetsrieder M
Publisher: American Chemical Society
Publication year: 2015
Volume: 87
Pages range: 6103-6111
ISSN: 0003-2700

Abstract

Heat sterilization of peritoneal dialysis (PD) fluids leads to partial degradation of the osmotic agent to form reactive carbonyl structures, which significantly reduce the biocompatibility of PD fluids and impair long-term PD therapy. Hence, it is important to know the exact composition of the degradation products to improve biocompatibility of PD fluids. Our study conducted targeted screening for degradation products in polyglucose (icodextrin)-containing PD fluids (pGDPs) by applying o-phenylenediamine (OPD) to form stable derivatives, which were analyzed by ultrahigh-performance liquid chromatography with hyphenated diode array tandem mass spectrometry (UHPLC−DAD−MS/MS). For the first time, specific degradation products of polyglucose, namely, 4-deoxyglucosone (4-DG) and 3,4-dideoxypentosone (3,4-DDPS), could be identified in PD fluids. Further, a reaction product of 5-hydroxymethylfurfural (5-HMF) and OPD could be characterized to be (5-(1H-benzo[d]imidazol-2-yl)furan-2-yl)methanol. Additionally, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal), both known to be present in glucose-based PD fluids, were also detected in polyglucose-containing fluids. Trapping a hitherto unknown degradation product with OPD yielded 1,4-bis(1H-benzo[d]imidazol-2-yl)-3,4-dihydroxybutan-1-one, which was present in heat- as well as filter-sterilized PD fluids.



How to cite
APA: Gensberger, S., Knabner, C., Waibel, R., Huppert, J., & Pischetsrieder, M. (2015). Qualitative profiling of polyglucose degradation products in peritoneal dialysis fluids. Analytical Chemistry, 87, 6103-6111. https://dx.doi.org/10.1021/acs.analchem.5b00665

MLA: Gensberger, Sabrina, et al. "Qualitative profiling of polyglucose degradation products in peritoneal dialysis fluids." Analytical Chemistry 87 (2015): 6103-6111.

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