Journal article


Development and validation of an HPLC method to quantify 3,4-dideoxyglucosone-3-ene in peritoneal dialysis fluids


Publication Details
Author(s): Frischmann M, Spitzer J, Fünfrocken M, Mittelmaier S, Deckert M, Fichert T, Pischetsrieder M
Publisher: Wiley-Blackwell
Publication year: 2009
Volume: 23
Pages range: 843-851
ISSN: 0269-3879

Abstract

A method was developed and validated to quantify 3,4-dideoxyglucosone-3-ene in peritoneal dialysis fluids by high-performance liquid chromatography with UV detection after derivatization with o-phenylenediamine. The advantages of this method compared with direct HPLC analysis are (i) the possibility of quantifying 3,4-dideoxyglucosone-3-ene simultaneously together with other glucose degradation products, (ii) the compatibility of the method with MS detection for unequivocal identification of the analyte and (iii) a bathochromic shift of the UV absorbance maximum which leads to higher selectivity. The validated method was used to measure 3,4-dideoxyglucosone-3-ene concentrations additionally to the glucose degradation products 3-deoxyglucosone, methylglyoxal, glyoxal, 5-hydroxymethylfurfural, 2-furaldehyde, formaldehyde and acetaldehyde in 19 commercial products for peritoneal dialysis. Copyright © 2009 John Wiley & Sons, Ltd.



How to cite
APA: Frischmann, M., Spitzer, J., Fünfrocken, M., Mittelmaier, S., Deckert, M., Fichert, T., & Pischetsrieder, M. (2009). Development and validation of an HPLC method to quantify 3,4-dideoxyglucosone-3-ene in peritoneal dialysis fluids. Biomedical Chromatography, 23, 843-851. https://dx.doi.org/10.1002/bmc.1194

MLA: Frischmann, Matthias, et al. "Development and validation of an HPLC method to quantify 3,4-dideoxyglucosone-3-ene in peritoneal dialysis fluids." Biomedical Chromatography 23 (2009): 843-851.

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