Journal article


Immunochemical Detection of Central Nervous Tissue in Retail Meat Products Using Myelin Proteolipid Protein (PLP) as Marker


Publication Details
Author(s): Hammon A, Düthorn T, Bäuerlein R, Becker CM, Pischetsrieder M, Pichner R, Gareis M
Publisher: Schlütersche Verlagsgesellschaft
Publication year: 2007
Volume: 58
Journal issue: 6
Pages range: 214-219
ISSN: 0003-925X

Abstract

A total of 152 different meat products (emulsion-type sausages, cooked sausages, fermented raw sausages) originating from local butchers shops and supermarkets were analysed for the presence of tissues from the central nervous system (CNS) by a newly developed Western blot assay using myelin proteolipid protein (PLP) as specific marker. Samples tested positive were analysed in parallel with a commercialized ELISA kit based on the detection of glial fibrillary acidic protein (GFAP). Analysing retail meat products by PL Passay, 9.9% of the samples (15 out of 152) showed positive PLP responses. The positive findings were confirmed in five cases (3.3 %) by application of the GFAP-ELISA. With both methods, very strong signals were detected in two sausages originating from a small butcher shop. Repeated sampling and analyses of these products during a period of several months proved the initial findings indicating a systematic contamination or addition of CNS material. The detection of nearly 10 % of CNS-positive sausages demonstrates that intentional or unintentional contaminations of retail meat products are not uncommon. Overall, the PLP assay was confirmed to be a highly sensitive method for the detection of CNS in both heat-treated and raw fermented sausages. Thus it could be applied for routine and food control purposes. © M.&H. Schaper GmbH&Co.



How to cite
APA: Hammon, A., Düthorn, T., Bäuerlein, R., Becker, C.-M., Pischetsrieder, M., Pichner, R., & Gareis, M. (2007). Immunochemical Detection of Central Nervous Tissue in Retail Meat Products Using Myelin Proteolipid Protein (PLP) as Marker. Archiv für Lebensmittelhygiene, 58(6), 214-219. https://dx.doi.org/10.2377/0003-925X-58-214

MLA: Hammon, Antje, et al. "Immunochemical Detection of Central Nervous Tissue in Retail Meat Products Using Myelin Proteolipid Protein (PLP) as Marker." Archiv für Lebensmittelhygiene 58.6 (2007): 214-219.

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