Journal article

Pyrodixal phosphate prevents progression of diabetic nephropathy

Publication Details
Author(s): Nakamura S, Li H, Adijiang A, Pischetsrieder M, Niwa T
Publisher: Oxford University Press (OUP): Policy B
Publication year: 2007
Volume: 22
Pages range: 2165-2174
ISSN: 0931-0509


Background. We have demonstrated that pyridoxal 50-phosphate (PLP), an active form of vitamin B6, inhibits formation of advanced glycation end-products (AGEs) by trapping 3-deoxyglucosone. The present study aimed to clarify if PLP could exert beneficial
effects on nephropathy in diabetic rats.
Methods. Streptozotocin (STZ)-induced diabetic rats were treated by oral administration of PLP or pyridoxamine (PM), another active form of vitamin B6, at a dose of 600 mg/kg/day for 16 weeks. AGEs [imidazolone, Ne-(carboxymethyl)lysine (CML) and N2-carboxyethyl-20-deoxyguanosine (CEdG)], transforming growth factor-b1 (TGF-b1), type 1 collagen
and fibronectin were detected in the kidneys using immunohistochemistry. Gene expression of TGF-b1 and receptor for AGEs (RAGEs) in the kidneys was determined using real-time quantitative polymerase chain reaction.
Results. Administration of PLP significantly inhibited albuminuria, glomerular hypertrophy, mesangial expansion, and interstitial fibrosis as compared with diabetic rats. PLP markedly inhibited accumulation of AGEs such as imidazolone, CML and CEdG, a DNAlinked
AGE, in glomeruli. PLP significantly inhibited expression of TGF-b1, type 1 collagen, fibronectin and RAGE in the kidneys. PLP was superior to PM in inhibiting accumulation of AGEs, expression of TGF-b1, type 1 collagen, and fibronectin, and the development of diabetic nephropathy.
Conclusions. PLP prevented progression of nephropathy in STZ-induced diabetic rats by inhibiting formation of AGEs. PLP is considered a promising active form of vitamin B6 for the treatment of AGElinked disorders such as diabetic nephropathy.

How to cite
APA: Nakamura, S., Li, H., Adijiang, A., Pischetsrieder, M., & Niwa, T. (2007). Pyrodixal phosphate prevents progression of diabetic nephropathy. Nephrology Dialysis Transplantation, 22, 2165-2174.

MLA: Nakamura, Sakurako, et al. "Pyrodixal phosphate prevents progression of diabetic nephropathy." Nephrology Dialysis Transplantation 22 (2007): 2165-2174.

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