Detection of central nervous system tissue in meat and meat products with a newly developed immunoassay selective for Myelin proteolipid protein

Sandmeier B, Bäuerlein R, Villmann C, Düthorn T, Gareis M, Becker CM, Pischetsrieder M (2007)

Publication Type: Journal article

Publication year: 2007

Journal

Food Chemistry Elsevier

Publisher: Elsevier

Book Volume: 105

Pages Range: 871-878

DOI: 10.1016/j.foodchem.2007.01.071

Abstract

Bovine spongiform encephalopathy (BSE) is most likely transmitted by the consumption of central nervous system (CNS) tissue of infected animals. In this study, an immunochemical assay for CNS in meat and meat products was developed using an antibody against Myelin proteolipid protein (PLP), which is very specifically expressed in the CNS. Solvent extraction of CNS-contaminated meat yielded a highly enriched PLP fraction. Subsequent Western blot analysis specifically detected the PLP band at 29 kDa. The detection limit for unprocessed CNS in raw meat was less than 0.025% and the quantification limit was calculated to be 0.049%. The PLP epitope was relatively stable during storage at 5 °C or -21 °C and during heating at 75 °C and 95 °C. Amounts of 0.1% CNS could be reliably detected in cooked bologna type sausage, cooked liver sausage and fermented sausage. Thus, the new assay allows highly specific and sensitive determination of CNS contaminations in meat and meat products. © 2007 Elsevier Ltd. All rights reserved.

Authors with CRIS profile

Cord-Michael Becker Lehrstuhl für Biochemie und Molekulare Medizin Monika Pischetsrieder Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)

Additional Organisation(s)

Emil-Fischer-Zentrum (Emil Fischer Center)

Involved external institutions

Max-Rubner-Institut - Bundesforschungsinstitut für Ernährung und Lebensmittel DE Germany (DE)

How to cite

APA:

Sandmeier, B., Bäuerlein, R., Villmann, C., Düthorn, T., Gareis, M., Becker, C.-M., & Pischetsrieder, M. (2007). Detection of central nervous system tissue in meat and meat products with a newly developed immunoassay selective for Myelin proteolipid protein. Food Chemistry, 105, 871-878. https://dx.doi.org/10.1016/j.foodchem.2007.01.071

MLA:

Sandmeier, Barbara, et al. "Detection of central nervous system tissue in meat and meat products with a newly developed immunoassay selective for Myelin proteolipid protein." Food Chemistry 105 (2007): 871-878.

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