Journal article


Two immunochemical assays to measure advanced glycation end-products in serum from dialysis patients


Publication Details
Author(s): Zhang X, Frischmann M, Kientsch-Engel R, Steinmann K, Stopper H, Niwa T, Pischetsrieder M
Publisher: Walter de Gruyter
Publication year: 2005
Volume: 43
Journal issue: 5
Pages range: 503-511
ISSN: 1434-6621

Abstract

Advanced glycation end-products are uremic toxins that accumulate in the serum and tissues of patients with chronic renal failure. Here, we established two enzyme-linked immunosorbent assays (ELISAs) for Nε-carboxymethyllysine and imidazolone to analyze advanced glycation end-products in human serum. Both ELISAs detected advanced glycation endproducts bound to human serum albumin in a dosedependent way. Whereas the formation of imidazolone was independent of the presence of oxygen, concentrations of Nε-carboxymethyllysine epitopes increased 20-fold under oxidative conditions. The Nε-carboxymethyllysine ELISA showed a similar response to free, peptide-bound and protein-bound Nε-carboxymethyllysine, whereas the imidazolone antibody showed slightly higher affinity toward peptide-bound compared to protein-bound imidazolone. In human serum, linear dilution ranges from 1:10 to 1:40 (Nε-carboxymethyllysine ELISA) and from 1:2 to 1:8 (imidazolone ELISA) were found. The recovery of Nε-carboxymethyllysine from serum was 101±10% and 94±12%, respectively, and 93±15% and 97±12% for imidazolone. The coefficients of variation for intraassay variability were 0.26-2.7% (Nε-carboxymethyllysine) and 0.1-2.4% (imidazolone), and 8.3-13.4% (Nε-carboxymethyllysine) and 7.8-12.5% (imidazolone) for inter-assay variability. In serum samples from hemodialysis patients (n = 20) and controls (n = 20), an approximately two-fold increase was detected in the patient group (p<0.001). The combination of the Nε-carboxymethyllysine and imidazolone ELISAs is a valuable tool to measure serum concentrations of advanced glycation end-products for clinical studies. © 2005 by Walter de Gruyter.



How to cite
APA: Zhang, X., Frischmann, M., Kientsch-Engel, R., Steinmann, K., Stopper, H., Niwa, T., & Pischetsrieder, M. (2005). Two immunochemical assays to measure advanced glycation end-products in serum from dialysis patients. Clinical Chemistry and Laboratory Medicine, 43(5), 503-511. https://dx.doi.org/10.1515/CCLM.2005.089

MLA: Zhang, Xiaohong, et al. "Two immunochemical assays to measure advanced glycation end-products in serum from dialysis patients." Clinical Chemistry and Laboratory Medicine 43.5 (2005): 503-511.

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