Journal article


In vitro formation of Nepsilon -(carboxymethyl)lysine and imidazolones under conditions similar to continous ambulatory peritoneal dialysis (CAPD)


Publication Details
Author(s): Tauer A, Knerr T, Niwa T, Schaub T, Lage C, Passlick-Deetjen J, Pischetsrieder M
Publisher: Elsevier
Publication year: 2001
Volume: 280
Pages range: 1408-1414
ISSN: 0006-291X

Abstract

Conventional peritoneal dialysis fluids (PDFs) lead to formation of advanced glycation end-products (AGE) in the peritoneal membrane. In this study, we investigated in vitro the dependence of AGE formation on regular changes of PDFs, as performed during continuous
ambulatory peritoneal dialysis (CAPD), and on the contribution of high glucose concentration versus glucose degradation products (GDPs). Under conditions similar to CAPD, protein glycating activity of a conventional single chamber bag PDF (CAPD 4.25%), two double chamber bag PDFs (CAPD Balance 4.25% and CAPD Bicarbonate 4.25%) and a sterile filtered
control was measured in vitro by Ne-(carboxymethyl) lysine (CML) and imidazolones, two well characterized, physiologically relevant AGE structures. Regular changes of PDFs increased AGE formation (CML 3.3-fold and imidazolone 2.6-fold) compared to incubation without changes. AGE formation by CAPD 4.25% was increased compared to control (imidazolones
7.9-fold and CML 3.3-fold) and the use of double chamber bag PDFs led to a decrease of imidazolones by 79% (CAPD Bicarbonate 4.25%) and by 66% (CAPD Balance 4.25%) and to CML contents similar to the control. These results indicate that a major part of AGEs were formed by GDPs in PDFs, whereas only a minor part was due to high glucose concentration. The use of double chamber bag fluids can reduce AGE formation considerably.



How to cite
APA: Tauer, A., Knerr, T., Niwa, T., Schaub, T., Lage, C., Passlick-Deetjen, J., & Pischetsrieder, M. (2001). In vitro formation of Nepsilon -(carboxymethyl)lysine and imidazolones under conditions similar to continous ambulatory peritoneal dialysis (CAPD). Biochemical and Biophysical Research Communications, 280, 1408-1414. https://dx.doi.org/10.1006/bbrc.2001.4294

MLA: Tauer, Andreas, et al. "In vitro formation of Nepsilon -(carboxymethyl)lysine and imidazolones under conditions similar to continous ambulatory peritoneal dialysis (CAPD)." Biochemical and Biophysical Research Communications 280 (2001): 1408-1414.

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