Immunochemical Detection of Oxalate Monoalkylamide, an Ascorbate-derived Maillard Reaction Product, in the Human Lens

Nagaraj RH, Shamsi F, Huber B, Pischetsrieder M (1999)

Publication Type: Journal article

Publication year: 1999

Journal

Febs Letters Elsevier

Publisher: Elsevier

Book Volume: 453

Pages Range: 327-330

DOI: 10.1016/S0014-5793(99)00571-2

Abstract

Carbohydrates with reactive aldehyde and ketone groups can undergo Maillard reactions with proteins to form advanced glycation end products. Oxalate monoalkylamide was identified as one of the advanced glycation end products formed from the Maillard reaction of ascorbate with proteins. In these experiments, we have analyzed human lens proteins immunochemically for the presence of oxalate monoalkylamide. Oxalate monoalkylamide was absent in most of the very young lenses but was present in old and cataractous lenses. The highest levels were found in senile brunescent lenses. Incubation experiments using bovine lens proteins revealed that oxalate monoalkylamide could form from the ascorbate degradation products, 2,3-diketogulonate and L-threose. These data provide the first evidence for oxalate monoalkylamide in vivo and suggest that ascorbate degradation and its binding to proteins are enhanced during lens aging and cataract formation.

Authors with CRIS profile

Monika Pischetsrieder

Involved external institutions

Case Western Reserve University US United States (USA) (US) Ludwig-Maximilians-Universität (LMU) DE Germany (DE)

How to cite

APA:

Nagaraj, R.H., Shamsi, F., Huber, B., & Pischetsrieder, M. (1999). Immunochemical Detection of Oxalate Monoalkylamide, an Ascorbate-derived Maillard Reaction Product, in the Human Lens. Febs Letters, 453, 327-330. https://dx.doi.org/10.1016/S0014-5793(99)00571-2

MLA:

Nagaraj, Ram H., et al. "Immunochemical Detection of Oxalate Monoalkylamide, an Ascorbate-derived Maillard Reaction Product, in the Human Lens." Febs Letters 453 (1999): 327-330.

BibTeX: Download