STXM goes 3D: Digital reconstruction of focal stacks as novel approach towards confocal soft x-ray microscopy
Author(s): Späth A, Schöll S, Rieß C, Schmidtel D, Paradossi G, Raabe J, Hornegger J, Fink R
Publication year: 2014
Pages range: 19-25
Fresnel zone plate based soft x-ray transmission microspectroscopy has developed into a routine technique for high-resolution elemental or chemical 2D imaging of thin film specimens. The availability of high resolution Fresnel lenses with short depth of focus offers the possibility of optical slicing (in the third dimension) by focus series with resolutions in the submicron regime. We introduce a 3D reconstruction algorithm that uses a variance-based metric to assign a focus measure as basis for volume rendering. The algorithm is applied to simulated geometries and opaque soft matter specimens thus enabling 3D visualization. These studies with z-resolution of few 100. nm serve as important step towards the vision of a confocal transmission x-ray microscope. © 2014 Elsevier B.V.
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APA: Späth, A., Schöll, S., Rieß, C., Schmidtel, D., Paradossi, G., Raabe, J.,... Fink, R. (2014). STXM goes 3D: Digital reconstruction of focal stacks as novel approach towards confocal soft x-ray microscopy. Ultramicroscopy, 144, 19-25. https://dx.doi.org/10.1016/j.ultramic.2014.04.004
MLA: Späth, Andreas, et al. "STXM goes 3D: Digital reconstruction of focal stacks as novel approach towards confocal soft x-ray microscopy." Ultramicroscopy 144 (2014): 19-25.