Fitzpatrick J, Kalyvas H, Filipovic M, Ivanovic-Burmazovic I, MacDonald JC, Shearer J, Kim E (2014)
Publication Type: Journal article, Original article
Publication year: 2014
Original Authors: Fitzpatrick J., Kalyvas H., Filipovic M.R., Ivanovic-Burmazovic I., Macdonald J.C., Shearer J., Kim E.
Publisher: American Chemical Society
Book Volume: 136
Pages Range: 7229-7232
Journal Issue: 20
DOI: 10.1021/ja5024207
Reversible modification of iron-sulfur clusters by nitric oxide acts as a genetic switch in a group of regulatory proteins. While the conversion of [Fe-S] clusters to iron-nitrosyls has been widely studied in the past, little is known about the reverse process, the repair of [Fe-S] clusters. Reported here is a system in which a mononitrosyl iron complex (MNIC), (PPN)[Fe(S Bu)(NO)] (1), is converted to a [2Fe-2S] cluster, (PPN)[FeS(SCHCH C(O)OMe)] (2). This conversion requires only the addition of a cysteine analogue, 3-mercaptomethylpropionate (MMP), at room temperature without the need for any other reagents. The identity of 2 was confirmed spectroscopically, chemically, crystallographically, and analytically. Mass spectrometry and S labeling studies support that the bridging sulfides in 2 derive from the added MMP, the cysteine analogue. The NO lost during the conversion of 1 to 2 is trapped in a dinitrosyl iron side product, (PPN)[Fe(SCHCHC(O)OMe)(NO)] (4). The present system implies that MNICs are likely intermediates in the repair of NO-damaged [2Fe-2S] clusters and that cysteine is a viable molecule responsible for the destabilization of MINCs and the formation of [2Fe-2S] clusters. © 2014 American Chemical Society.
APA:
Fitzpatrick, J., Kalyvas, H., Filipovic, M., Ivanovic-Burmazovic, I., MacDonald, J.C., Shearer, J., & Kim, E. (2014). Transformation of a mononitrosyl iron complex to a [2Fe-2S] cluster by a cysteine analogue. Journal of the American Chemical Society, 136(20), 7229-7232. https://dx.doi.org/10.1021/ja5024207
MLA:
Fitzpatrick, Jessica, et al. "Transformation of a mononitrosyl iron complex to a [2Fe-2S] cluster by a cysteine analogue." Journal of the American Chemical Society 136.20 (2014): 7229-7232.
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