Loss of eyes in zebrafish caused by mutation of chokh/rx 3
Loosli F, Staub W, Finger-Baier KC, Ober EA, Verkade H, Wittbrodt J, Baier H (2003)
Publication Language: English
Publication Type: Journal article
Publication year: 2003
Journal
Book Volume: 4
Pages Range: 894-899
Journal Issue: 9
DOI: 10.1038/sj.embor.embor919
Abstract
The vertebrate eye forms by specification of the retina anlage and subsequent morphogenesis of the optic vesicles, from which the neural retina differentiates. chokh (chk) mutant zebrafish lack eyes from the earliest stages in development. Marker gene analysis indicates that retinal fate is specified normally, but optic vesicle evagination and neuronal differentiation are blocked. We show that the chk gene encodes the homeodomain-containing transcription factor, Rx3. Loss of Rx3 function in another teleost, medaka, has also been shown to result in an eyeless phenotype. The medaka rx3 locus can fully rescue the zebrafish mutant phenotype. We provide evidence that the regulation of rx3 is evolutionarily conserved, whereas the downstream cascade contains significant differences in gene regulation. Thus, these mutations in orthologous genes allow us to study the evolution of vertebrate eye development at the molecular level.
Authors with CRIS profile
Involved external institutions
European Molecular Biology Laboratory (EMBL)
Germany (DE)
University of California San Francisco (UCSF)
United States (USA) (US)
Germany (DE)
University of California San Francisco (UCSF)
United States (USA) (US)
How to cite
APA:
Loosli, F., Staub, W., Finger-Baier, K.C., Ober, E.A., Verkade, H., Wittbrodt, J., & Baier, H. (2003). Loss of eyes in zebrafish caused by mutation of chokh/rx 3. EMBO Reports, 4(9), 894-899. https://doi.org/10.1038/sj.embor.embor919
MLA:
Loosli, Felix, et al. "Loss of eyes in zebrafish caused by mutation of chokh/rx 3." EMBO Reports 4.9 (2003): 894-899.
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