A green approach to encapsulate proteins and enzymes within crystalline lanthanide-based Tb and Gd MOFs

Tocco D, Joshi M, Mastrangelo R, Fratini E, Salis A, Hartmann M (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Dalton Transactions Royal Society of Chemistry

DOI: 10.1039/d4dt01667j

Abstract

In this work, bovine serum albumin (BSA) and Aspergillus sp. laccase (LC) were encapsulated in situ within two lanthanide-based MOFs (TbBTC and GdBTC) through a green one-pot synthesis (almost neutral aqueous solution, T = 25 °C, and atmospheric pressure) in about 1 h. Pristine MOFs and protein-encapsulated MOFs were characterized through wide angle X-ray scattering, scanning electron microscopy, thermogravimetric analysis, Fourier transform infrared and Raman spectroscopies. The location of immobilized BSA molecules, used as a model protein, was investigated through small angle X-ray scattering. BSA occurs both on the inner and on the outer surface of the MOFs. LC@TbBTC, and LC@GdBTC samples were also characterized in terms of specific activity, kinetic parameters, and storage stability both in water and acetate buffer. The specific activity of LC@TbBTC was almost twice that of LC@GdBTC (10.8 μmol min−1 mg−1vs. 6.6 μmol min−1 mg−1). Both biocatalysts showed similar storage stabilities retaining ∼60% of their initial activity after 7 days and ∼20% after 21 days. LC@TbBTC dispersed in acetate buffer exhibited a higher storage stability than LC@GdBTC. Additionally, terbium-based MOFs showed interesting luminescent properties. Together, these findings suggest that TbBTC and GdBTC are promising supports for the in situ immobilization of proteins and enzymes.

Authors with CRIS profile

Madhura Joshi Sonderforschungsbereich 1411 Produktgestaltung disperser Systeme / Design of Particulate Products Martin Hartmann Professur für Katalyse

Additional Organisation(s)

Interface Research and Catalysis (ECRC)

Involved external institutions

Università degli Studi di Cagliari IT Italy (IT) Università degli Studi di Firenze / University of Florence IT Italy (IT)

How to cite

APA:

Tocco, D., Joshi, M., Mastrangelo, R., Fratini, E., Salis, A., & Hartmann, M. (2024). A green approach to encapsulate proteins and enzymes within crystalline lanthanide-based Tb and Gd MOFs. Dalton Transactions. https://doi.org/10.1039/d4dt01667j

MLA:

Tocco, Davide, et al. "A green approach to encapsulate proteins and enzymes within crystalline lanthanide-based Tb and Gd MOFs." Dalton Transactions (2024).

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