Nanoscale Topography of Anodic TiO2 Nanostructures Is Crucial for Cell-Surface Interactions

Park J, Tesler A, Gongadze E, Iglič A, Schmuki P, Mazare AV (2024)

Publication Type: Journal article

Publication year: 2024

Journal

ACS Applied Materials and Interfaces American Chemical Society

Book Volume: 16

Pages Range: 4430-4438

Journal Issue: 4

DOI: 10.1021/acsami.3c16033

Abstract

Anodic titanium dioxide (TiO2) nanostructures, i.e., obtained by electrochemical anodization, have excellent control over the nanoscale morphology and have been extensively investigated in biomedical applications owing to their sub-100 nm nanoscale topography range and beneficial effects on biocompatibility and cell interactions. Herein, we obtain TiO2 nanopores (NPs) and nanotubes (NTs) with similar morphologies, namely, 15 nm diameter and 500 nm length, and investigate their characteristics and impact on stem cell adhesion. We show that the transition of TiO2 NPs to NTs occurs via a pore/wall splitting mechanism and the removal of the fluoride-rich layer. Furthermore, in contrast to the case of NPs, we observe increased cell adhesion and proliferation on nanotubes. The enhanced mesenchymal stem cell adhesion/proliferation seems to be related to a 3-fold increase in activated integrin clustering, as confirmed by immunogold labeling with β1 integrin antibody on the nanostructured layers. Moreover, computations of the electric field and surface charge density show increased values at the inner and outer sharp edges of the top surfaces of the NTs, which in turn can influence cell adhesion by increasing the bridging interactions mediated by proteins and molecules in the environment. Collectively, our results indicate that the nanoscale surface architecture of the lateral spacing topography can greatly influence stem cell adhesion on substrates for biomedical applications.

Authors with CRIS profile

Alexander Tesler Lehrstuhl für Werkstoffwissenschaften (Korrosion und Oberflächentechnik) Patrik Schmuki Lehrstuhl für Werkstoffwissenschaften (Korrosion und Oberflächentechnik) Anca Valentina Mazare Lehrstuhl für Werkstoffwissenschaften (Korrosion und Oberflächentechnik)

Involved external institutions

University of Ljubljana (UL) / Univerza v Ljubljani SI Slovenia (SI)

How to cite

APA:

Park, J., Tesler, A., Gongadze, E., Iglič, A., Schmuki, P., & Mazare, A.V. (2024). Nanoscale Topography of Anodic TiO2 Nanostructures Is Crucial for Cell-Surface Interactions. ACS Applied Materials and Interfaces, 16(4), 4430-4438. https://doi.org/10.1021/acsami.3c16033

MLA:

Park, Jung, et al. "Nanoscale Topography of Anodic TiO2 Nanostructures Is Crucial for Cell-Surface Interactions." ACS Applied Materials and Interfaces 16.4 (2024): 4430-4438.

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