Direct 3D‐Bioprinting of hiPSC‐derived Cardiomyocytes to Generate Functional Cardiac Tissues

Esser T, Anspach A, Muenzebrock K, Kah DTE, Schrüfer S, Schenk J, Heinze KG, Schubert DW, Fabry B, Engel F (2023)

Publication Type: Journal article

Publication year: 2023

Journal

DOI: 10.1002/adma.202305911

Abstract

3D-bioprinting is a promising technology to produce human tissues as drug screening tool or for organ repair. However, direct printing of living cells has proven difficult. Here, we present a method to directly 3D-bioprint human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes embedded in a collagen-hyaluronic acid ink generating centimeter-sized functional ring- and ventricle-shaped cardiac tissues in an accurate and reproducible manner. The printed tissues contained hiPSC-derived cardiomyocytes with well-organized sarcomeres and exhibited spontaneous and regular contractions, which persisted for several months and were able to contract against passive resistance. Importantly, beating frequencies of the printed cardiac tissues could be modulated by pharmacological stimulation. This approach opens up new possibilities for generating complex functional cardiac tissues as models for advanced drug screening or as tissue grafts for organ repair or replacement.

Authors with CRIS profile

Involved external institutions

Julius-Maximilians-Universität Würzburg Germany (DE)

How to cite

APA:

Esser, T., Anspach, A., Muenzebrock, K., Kah, D.-T.E., Schrüfer, S., Schenk, J.,... Engel, F. (2023). Direct 3D‐Bioprinting of hiPSC‐derived Cardiomyocytes to Generate Functional Cardiac Tissues. Advanced Materials. https://doi.org/10.1002/adma.202305911

MLA:

Esser, Tilman, et al. "Direct 3D‐Bioprinting of hiPSC‐derived Cardiomyocytes to Generate Functional Cardiac Tissues." Advanced Materials (2023).

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