Amorphous Calcium Phosphate and Amorphous Calcium Phosphate Carboxylate: Synthesis and Characterization

Indurkar A, Choudhary R, Rubenis K, Nimbalkar M, Sarakovskis A, Boccaccini AR, Locs J (2023)

Publication Type: Journal article

Publication year: 2023

Journal

ACS Omega American Chemical Society: Open Access Titles / American Chemical Society

Book Volume: 8

Pages Range: 26782-26792

Journal Issue: 30

DOI: 10.1021/acsomega.3c00796

Abstract

Amorphous calcium phosphate (ACP) is the first solid phase precipitated from a supersaturated calcium phosphate solution. Naturally, ACP is formed during the initial stages of biomineralization and stabilized by an organic compound. Carboxylic groups containing organic compounds are known to regulate the nucleation and crystallization of hydroxyapatite. Therefore, from a biomimetic point of view, the synthesis of carboxylate ions containing ACP (ACPC) is valuable. Usually, ACP is synthesized with fewer steps than ACPC. The precipitation reaction of ACP is rapid and influenced by pH, temperature, precursor concentration, stirring conditions, and reaction time. Due to phosphates triprotic nature, controlling pH in a multistep approach becomes tedious. Here, we developed a new ACP and ACPC synthesis approach and thoroughly characterized the obtained materials. Results from vibration spectroscopy, nuclear magnetic resonance (NMR), X-ray photoelectron spectroscopy (XPS), true density, specific surface area, and ion release studies have shown a difference in the physiochemical properties of the ACP and ACPC. Additionally, the effect of a carboxylic ion type on the physiochemical properties of ACPC was characterized. All of the ACPs and ACPCs were synthesized in sterile conditions, and in vitro analysis was performed using MC-3T3E1 cells, revealing the cytocompatibility of the synthesized ACPs and ACPCs, of which the ACPC synthesized with citrate showed the highest cell viability.

Authors with CRIS profile

Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Riga Technical University LV Latvia (LV) Shivaji University / சிவாஜி பல்கலைக்கழகம் IN India (IN) University of Latvia LV Latvia (LV)

How to cite

APA:

Indurkar, A., Choudhary, R., Rubenis, K., Nimbalkar, M., Sarakovskis, A., Boccaccini, A.R., & Locs, J. (2023). Amorphous Calcium Phosphate and Amorphous Calcium Phosphate Carboxylate: Synthesis and Characterization. ACS Omega, 8(30), 26782-26792. https://dx.doi.org/10.1021/acsomega.3c00796

MLA:

Indurkar, Abhishek, et al. "Amorphous Calcium Phosphate and Amorphous Calcium Phosphate Carboxylate: Synthesis and Characterization." ACS Omega 8.30 (2023): 26782-26792.

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