Preparation and characterization of novel poly (lactic acid)/calcium oxide nanocomposites by electrospinning as a potential bone tissue scaffold
Canales D, Moyano D, Alvarez F, Grande-Tovar CD, Valencia-Llano CH, Peponi L, Boccaccini AR, Zapata PA (2023)
Publication Type: Journal article
Publication year: 2023
Journal
Book Volume: 153
Article Number: 213578
DOI: 10.1016/j.bioadv.2023.213578
Abstract
Calcium oxide nanoparticles (n-CaO) ca. 22 nm were obtained from eggshell waste. The n-CaO was incorporated into the PLA matrix in 10 and 20 wt% of filler content by electrospinning process to get PLA/n-CaO fibers with homogenous morphology and diameter as a potential use in scaffold for bone tissue regeneration. The incorporation of n-CaO into PLA modifies the mechanical properties, having a reinforcement effect on the matrix. The Young modulus for PLA/n-CaO nanocomposites increased between 122 and 138 % concerning neat PLA fibers, showing a more rigid behavior. The PLA/n-CaO nanocomposite fibers showed in vitro bioactivity, capable of inducing the precipitation of hydroxyapatite (HA) layer in the fiber surface after seven days in SBF solution. The biocidal and biological properties of PLA/n-Cao with 20 wt% showed a 30 % reduction in bacterial viability against S. aureus and 11 % against E. coli after 6 h of bacterial exposure. Furthermore, the fibers did not show a cytotoxic effect on the bone marrow ST-2 cell line, allowing cell adhesion and proliferation in the RPMI medium. The PLA/n-CaO with 20 wt% of nanoparticles showed a higher capacity to promote osteogenic differentiation, significantly increasing the alkaline phosphatase (ALP) expression after seven days compared to PLA and cell control. The in vivo analysis corroborated the biocompatibility of the prepared scaffolds; the presence of n-CaO in PLA reduced the formation of fibrous encapsulation of the material, improving the healing process. These results validated using n-CaO to enhance the functionality of polymer matrices as a PLA, bringing bioactive, biocide, and biocompatible properties, opening a new and interesting route to develop new biomaterials as a scaffold for bone tissue engineering.
Authors with CRIS profile
Involved external institutions
Universidad de Santiago de Chile (USACH) / University of Santiago, Chile
Chile (CL)
University of Atlántico / Universidad del Atlántico (UA)
Colombia (CO)
Spanish National Research Council / Consejo Superior de Investigaciones Científicas (CSIC)
Spain (ES)
Chile (CL)
University of Atlántico / Universidad del Atlántico (UA)
Colombia (CO)
Spanish National Research Council / Consejo Superior de Investigaciones Científicas (CSIC)
Spain (ES)
How to cite
APA:
Canales, D., Moyano, D., Alvarez, F., Grande-Tovar, C.D., Valencia-Llano, C.H., Peponi, L.,... Zapata, P.A. (2023). Preparation and characterization of novel poly (lactic acid)/calcium oxide nanocomposites by electrospinning as a potential bone tissue scaffold. Biomaterials Advances, 153. https://dx.doi.org/10.1016/j.bioadv.2023.213578
MLA:
Canales, Daniel, et al. "Preparation and characterization of novel poly (lactic acid)/calcium oxide nanocomposites by electrospinning as a potential bone tissue scaffold." Biomaterials Advances 153 (2023).
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