Neutrophil Extracellular Traps Drive Dacryolithiasis

Zlatar L, Timm T, Lochnit G, Bilyy R, Bäuerle T, Munoz Becerra M, Schett G, Knopf J, Heichel J, Ali MJ, Schapher M, Paulsen F, Herrmann M (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Cells MDPI

Book Volume: 12

Article Number: 1857

Journal Issue: 14

DOI: 10.3390/cells12141857

Abstract

Mucopeptide concretions, previously called dacryoliths, are macroscopic stones that commonly obstruct the lacrimal sac. The mechanism behind dacryolithiasis remains unclear; however, the involvement of various immune cells, including neutrophils, has been confirmed. These findings remain limited, and no information on neutrophil extracellular traps (NETs), essentially involved in the pathogenesis of other lithiases, is available yet. Here, we employ microcomputed tomography, magnetic resonance tomography, histochemistry, mass spectrometry, and enzyme activity analyses to investigate the role of neutrophils and NETs in dacryolithiasis. We classify mucopeptide concretions into three types, with respect to the quantity of cellular and acellular material, polysaccharides, and mucosubstances. We propose the role of neutrophils and NETs within the existing model of gradual formation and growth of mucopeptide concretions, with neutrophils contributing to the initial stages of dacryolithiasis, as they localized on the inner (older) parts of the tissue. As NETs localized on the outer (newer) parts of the tissue, we link their role to the late stages of dacryolithiasis, presumably maintaining the proinflammatory environment and preventing efficient clearance. An abundance of IgG on the surface indicates the involvement of the adaptive immune system later as well. These findings bring new perspectives on dacryolithiasis, in which the innate and adaptive immune system are essentially involved.

Authors with CRIS profile

Leticija Zlatar Department of Medicine 3 – Rheumatology and Immunology Tobias Bäuerle Professur für Multimodale Bildgebung in der präklinischen Forschung Marco Munoz Becerra Department of Medicine 3 – Rheumatology and Immunology Georg Schett Lehrstuhl für Innere Medizin III Jasmin Knopf Department of Medicine 3 – Rheumatology and Immunology Mirco Schapher Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie Martin Herrmann Department of Medicine 3 – Rheumatology and Immunology

Involved external institutions

Martin-Luther-Universität Halle-Wittenberg (MLU) DE Germany (DE) Justus-Liebig-Universität Gießen DE Germany (DE) L V Prasad Eye Institute IN India (IN) Danylo Halytsky Lviv National Medical University / Львiвський Національний Медичний Унiверситет iм. Данила Галицького UA Ukraine (UA)

How to cite

APA:

Zlatar, L., Timm, T., Lochnit, G., Bilyy, R., Bäuerle, T., Munoz Becerra, M.,... Herrmann, M. (2023). Neutrophil Extracellular Traps Drive Dacryolithiasis. Cells, 12(14). https://doi.org/10.3390/cells12141857

MLA:

Zlatar, Leticija, et al. "Neutrophil Extracellular Traps Drive Dacryolithiasis." Cells 12.14 (2023).

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