A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows
Kahl M, Schneidereit D, Meinert C, Bock N, Hutmacher DW, Friedrich O (2023)
Publication Type: Journal article, Original article
Publication year: 2023
Journal
Book Volume: 14
Article Number: 100372
DOI: 10.1016/j.biosx.2023.100372
Abstract
Cost-effective automated solutions for hydrogel-based 3D cell culture workflows are required to increase throughput, reproducibility and user-independent results. 3D bioprinters can handle viscous biomaterials, however, throughput is low, they are limited to specific biomaterials, and lack post-processing and analysis methods. Conversely, liquid handling robots have higher throughput but miss viscous materials handling capabilities. Furthermore, commercial systems are not only expensive but mostly not ‘open-source’ and hence are not adaptable to specific experimental requirements. The aim of this study was the implementation of an opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows from production to analysis into a previously developed biomanufacturing workstation. An analysis module with a fluorescence wide-field microscope and a motorised XYZ stage was engineered to transport tissue-culture plates from the storage rack of the workstation to the custom-made microscope and to assess fluorescence-based cell parameters. The microscope has two fluorescence channels and a resolution greater than 228 lp/mm. The XYZ-stage achieved an accuracy of 0.8 μm in X-direction (repeatability 2.4 μm) and 1.4 μm in Z-direction (repeatability <0.1 μm). Viability assays and drug-response studies were conducted on 2D and 3D prostate cancer gelatin-methacryloyl hydrogel models demonstrating its capabilities comparable to a conventional fluorescence microscope. Altogether, the integration of an opto-mechatronic screening module into the biomanufacturing workstation enables automated, reproducible, and user-independent production, imaging and analysis of hydrogel-based 3D cell cultures.
Authors with CRIS profile
Melanie Kahl
Lehrstuhl für Medizinische Biotechnologie
Dominik Schneidereit
Lehrstuhl für Medizinische Biotechnologie
Oliver Friedrich
Lehrstuhl für Medizinische Biotechnologie
Involved external institutions
Australia (AU)How to cite
APA:
Kahl, M., Schneidereit, D., Meinert, C., Bock, N., Hutmacher, D.W., & Friedrich, O. (2023). A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows. Biosensors and Bioelectronics: X, 14. https://dx.doi.org/10.1016/j.biosx.2023.100372
MLA:
Kahl, Melanie, et al. "A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows." Biosensors and Bioelectronics: X 14 (2023).
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