A comparative in vitro and in vivo analysis of the biological properties of the 45S5-, 1393-, and 0106-B1-bioactive glass compositions using human bone marrow-derived stromal cells and a rodent critical size femoral defect model

Kunisch E, Fiehn LA, Saur M, Arango Ospina M, Merle C, Hagmann S, Stiller A, Hupa L, Renkawitz T, Boccaccini AR, Westhauser F (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Biomaterials Advances Elsevier

Book Volume: 153

Article Number: 213521

DOI: 10.1016/j.bioadv.2023.213521

Abstract

Since the introduction of the 45S5-bioactive glass (BG), numerous new BG compositions have been developed. Compared to the 45S5-BG, 1393-BG shows favorable processing properties due to its low crystallization tendency and the 1393-BG-based borosilicate 0106-B1-BG exhibits improved angiogenic properties due to its boron content. Despite their close (chemical) relationship, the biological properties of the mentioned BG composition have not yet been comparatively examined. In this study, the effects of the BGs on proliferation, viability, osteogenic differentiation, and angiogenic factor production of human bone marrow-derived mesenchymal stromal cells were assessed. Scaffolds made of the BGs were introduced in a critical-sized femur defect model in rats in order to analyze their impact on bone defect regeneration. In vitro, 1393-BG and 0106-B1-BG outperformed 45S5-BG with regard to cell proliferation and viability. 1393-BG enhanced osteogenic differentiation; 0106-B1-BG promoted angiogenic factor production. In vivo, 0106-B1-BG and 45S5-BG outperformed 1393-BG in terms of angiogenic and osteoclastic response resulting in improved bone regeneration. In conclusion, the biological properties of BGs can be significantly modified by tuning their composition. Demonstrating favorable processing properties and an equally strong in vivo bone regeneration potential as 45S5-BG, 0106-B1-BG qualifies as a basis to incorporate other bioactive ions to improve its biological properties.

Authors with CRIS profile

Marcela Arango Ospina Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Universitätsklinikum Heidelberg DE Germany (DE) Åbo Akademi University FI Finland (FI)

How to cite

APA:

Kunisch, E., Fiehn, L.A., Saur, M., Arango Ospina, M., Merle, C., Hagmann, S.,... Westhauser, F. (2023). A comparative in vitro and in vivo analysis of the biological properties of the 45S5-, 1393-, and 0106-B1-bioactive glass compositions using human bone marrow-derived stromal cells and a rodent critical size femoral defect model. Biomaterials Advances, 153. https://dx.doi.org/10.1016/j.bioadv.2023.213521

MLA:

Kunisch, Elke, et al. "A comparative in vitro and in vivo analysis of the biological properties of the 45S5-, 1393-, and 0106-B1-bioactive glass compositions using human bone marrow-derived stromal cells and a rodent critical size femoral defect model." Biomaterials Advances 153 (2023).

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